Study M1024
Study name | Fan L 2023 |
Title | Brain regional pharmacokinetics of hydroxytyrosol and its molecular mechanism against depression assessed by multi-omics approaches |
Overall design | The aim of this study was to reveal the specific antidepressive mechanism of hydroxytyrosol in chronic unpredictable mild stress (CUMS) induced depressive mice. ICR mice were divided into the following 3 groups (n = 9 in each group): (1) control group, (2) CUMS group, and (3) CUMS + hydroxytyrosol group (stressor plus hydroxytyrosol treatment at the dose of 70 mg/kg). The CUMS stress procedure lasted for 4 weeks, and drugs were administered via intragastric once per day during the model building period. Serum samples were used for untargeted metabonomic analysis (n = 8/group), and metabolic profiles in the different micro-regions (CA1, CA3 and DG regions) of the hippocampus were investigated using spatial-resolved metabolomics (n = 3/group). |
Study Type | Type1; Type2; |
Data available | Unavailable |
Organism | Mouse; ICR mouse; |
Categories of depression | Animal model; Chronic mild stress model; Chronic mild stress model; |
Criteria for depression | Sucrose preference test, forced swimming test, tail suspension test |
Sample size | 24 |
Tissue | Central; Brain; CA3 region of hippocampus; Peripheral; Blood; Serum; Central; Brain; CA1 region of hippocampus; Central; Brain; DG region of hippocampus; |
Platform | MS-based; LC-MS: Dionex U3000 UPLC system with QE plus high-resolution mass spectrometer (Thermo Fisher Scientific, USA); MS-based; AFADESI-MSI: Airflow-assisted desorption electrospray ionization mass spectrometry imaging; |
PMID | |
DOI | |
Citation | Fan L, Peng Y, Li X. Brain regional pharmacokinetics of hydroxytyrosol and its molecular mechanism against depression assessed by multi-omics approaches. Phytomedicine. 2023 Apr;112:154712. |
Metabolite | Citric acid; Linoleic acid; Stearic acid; Myristic acid; Palmitoleic acid; Palmitic acid; N-Acetyl-L-aspartic acid; L-Tryptophan; Arachidonic acid; D-Glucose; L-Arginine; Sebacic acid; Bilirubin; Cholic acid; Propionylcarnitine; Oxoglutaric acid; Indoxyl sulfate; Ascorbic acid; Taurine; Betaine; Creatine; Histamine; Docosahexaenoic acid; Adenosine; L-Acetylcarnitine; Glycerophosphocholine; L-Carnitine; LysoPC(18:1); LysoPC(18:2); LysoPC(20:1); Oleoylcarnitine; Stearoylcarnitine; D-Galactose; LysoPC(16:1/0:0); Dodecanoylcarnitine; Benzoic acid; 4-Trimethylammoniobutanoic acid; Spermidine; PE(18:1/0:0); Corticosterone; Hydroxybutyrylcarnitine; L-Palmitoylcarnitine; Proline betaine; LysoPC(20:4); LysoPC(20:5); LysoPC(22:6); Alpha-Linolenic acid; LysoPE(0:0/20:1); Cer(d18:1/16:0); Monoethylhexyl phthalic acid; LysoPC(22:5); LysoPC(20:3); Sphinganine; LysoPE(16:0/0:0); PC(15:0/0:0); PC(18:1/0:0); PC(20:5/0:0); Dodecanoic acid; Eicosapentaenoic acid; LysoPC(22:1); LysoPC(14:0/0:0); LysoPE(0:0/18:2); LysoPE(18:1/0:0); LysoPE(0:0/22:4); LysoPC(20:2); Myristoleic acid; LysoPC(18:3); LysoPC(0:0/16:0); PS(10:0/10:0); Isovaleraldehyde; N-Undecanoylglycine; 12,13-DHOME; LysoPE(22:6/0:0); LysoPE(18:2/0:0); SM(d17:1/17:0); Tetracosahexaenoic acid; Galactaric acid; PC(22:4/0:0); PC(22:6/0:0); PC(0:0/20:4); PC(22:5/0:0); PC(19:1/0:0); PC(20:3/0:0); LysoPE(0:0/20:3); LysoPE(23:0/0:0); PE(20:2/0:0); MIPPC(t18:0/22:0(2OH)); OHHdiA-PE; PE(20:1/0:0); PC(20:1/0:0); PC(O-18:2/2:0); PC(20:2/0:0); PC(10:0/9:0); PC(P-18:0/0:0); LysoPE(0:0/22:6); LysoPE(0:0/22:5); PIM1(19:0/16:0); LysoPE(22:5/0:0); LysoPE(16:1/0:0); LysoPE(0:0/16:1); PIM1(19:1/18:1); LysoPE(0:0/20:4); LysoPE(20:4/0:0); PIM1(19:2/18:0); PA(21:4/0:0); PE(0:0/22:4); PIM1(17:0/18:0); PC(16:0/0:0); PE(18:0/0:0); PC(O-14:0/2:0); PE(0:0/18:0); PC(16:1/0:0); PC(17:1/0:0); PC(18:3/0:0); PC(0:0/14:0); PI(20:4/0:0); PHOHA-PA; PI(18:2/0:0); |
