Study M068
Study name | Yu XY 2015 |
Title | A urinary metabolomics study of the metabolic dysfunction and the regulation effect of citalopram in rats exposed to chronic unpredictable mild stress |
Overall design | In this study, an untargeted metabolic profiling based on Ultra High Performance Liquid Chromatography coupled with Orbitrap mass spectrometry (UPLC-Orbitrap-MS) has been performed to investigate the urinary metabolic changes between chronic unpredictable mild stress (CUMS) in rats and healthy control rats, and screen for drug efficacy on these changed metabolites after administration of citalopram, then provide a new perspective into the understanding of the antidepressant effects of citalopram. Rats were divided into 3 groups (n = 6 in each group): (1) control group, (2) CUMS group, and (3) CUMS + citalopram group (stress plus citalopram treatment at the dose of 10 mg/kg). The CUMS stress procedure lasted for 6 weeks, and citalopram was administered intraperitoneally during the last 3 weeks of the model building period. |
Study Type | Type1; Type2; |
Data available | Unavailable |
Organism | Rat; Sprague-Dawley rat; |
Categories of depression | Animal model; Chronic mild stress model; Chronic mild stress model; |
Criteria for depression | Sucrose preference test, forced swimming test |
Sample size | 18 |
Tissue | Peripheral; Urine; Urine; |
Platform | MS-based; LC-MS: UPLC Ultimate 3000 system (Dionex, Germering, Germany), coupled to an Orbitrap mass spectrometer (Thermo Fisher Scientific, Bremen, Germany); |
PMID | |
DOI | |
Citation | Yu X, Luo J, Chen L, et al. A urinary metabolomics study of the metabolic dysfunction and the regulation effect of citalopram in rats exposed to chronic unpredictable mild stress. RSC Adv 2015;5, 69800-12. |
Metabolite | Gamma-Aminobutyric acid; Citric acid; Glycerol; N-Acetyl-L-aspartic acid; L-Phenylalanine; L-Tryptophan; Glycine; L-Serine; L-Isoleucine; Succinic acid; 5-Hydroxyindoleacetic acid; Phenylpyruvic acid; L-Kynurenine; Kynurenic acid; Phenylacetylglycine; Xanthurenic acid; Indoleacetic acid; Indoxyl sulfate; |
