Study M732
Study name | Hao CY 2020 |
Title | Intravenous administration of sodium propionate induces antidepressant or prodepressant effect in a dose dependent manner |
Overall design | The aim of this study was to investigate the effects of propionate on depression. Propionate dose gradients were intravenously administrated to rats exposed to chronic unpredictable mild stress (CUMS) for 1 week. Sprague-Dawley rats were divided into the following 5 groups (n = 16 in each group): (1) control group, (2) CUMS group, (3) CUMS + low dose of propionate group (stressor plus propionate treatment at the dose of 2 mg/kg), (4) CUMS + middle dose of propionate group (stressor plus propionate treatment at the dose of 20 mg/kg), and (5) CUMS + high dose of propionate group (stressor plus propionate treatment at the dose of 200 mg/kg). The CUMS stress procedure lasted for 4 weeks, and drugs were administered via intravenously once per day for 1 week after stress. Results of these behavioral tests demonstrate that low dose propionate (2 mg/kg body weight/day) induces antidepressant effect through body weight recovery, elevated reward-seeking behaviors, and reduced depression-like behaviors, while high dose propionate (200 mg/kg body weight/day) induces prodepressant effects opposite of those of low dose propionate. Neurotransmitters in the hippocampus of each rat was quantified by UPLC-MS/MS. Hippocampus metabolome profiling was performed by GC-MS/MS. |
Study Type | Type1; Type2; |
Data available | Unavailable |
Organism | Rat; Sprague-Dawley rat; |
Categories of depression | Animal model; Chronic mild stress model; Chronic mild stress model; |
Criteria for depression | Sucrose preference test, forced swimming test, tail suspension test |
Sample size | 80 |
Tissue | Central; Brain; Hippocampus; |
Platform | MS-based; GC-MS: trace gas chromatograph coupled with a Polyris Q Ion Trap mass spectrometer (Thermo Fisher Scientific, MA, USA); MS-based; LC-MS: Thermo Scientific Dionex Ultimate 3000 RSLC system combined with a Thermo Q Executive Orbitrap mass spectrometer; |
PMID | |
DOI | |
Citation | Hao C, Gao Z, Liu X, et al. Intravenous administration of sodium propionate induces antidepressant or prodepressant effect in a dose dependent manner. Sci Rep. 2020 Nov 16;10(1):19917. |
Metabolite | Gamma-Aminobutyric acid; L-Phenylalanine; L-Tryptophan; L-Glutamic acid; L-Serine; L-Aspartic acid; Homovanillic acid; L-Tyrosine; L-Kynurenine; Kynurenic acid; 3,4-Dihydroxybenzeneacetic acid; Serotonin; Hydroxykynurenine; Norepinephrine; Dopamine; Quinolinic acid; |
