Study M540
Study name | Geng CM 2019b |
Title | Integrated metabolomics and lipidomics profiling of hippocampus reveal metabolite biomarkers in a rat model of chronic unpredictable mild stress-induced depression |
Overall design | The aim of this study was to conduct integrated metabolomics and lipidomics to identify metabolites and lipids biomarkers in the hippocampus in rat models of chronic unpredictable mild stress (CUMS)-induced depression. Sprague-Dawley rats were divided into the following 2 groups (n = 6 in each group): (1) control group, and (2) CUMS group. The CUMS stress procedure lasted for 4 weeks. Hippocampal samples per group were used for metabonomics and lipidomics analyses. |
Study Type | Type1; |
Data available | Unavailable |
Organism | Rat; Sprague-Dawley rat; |
Categories of depression | Animal model; Chronic mild stress model; Chronic mild stress model; |
Criteria for depression | Sucrose preference test, forced swimming test |
Sample size | 12 |
Tissue | Central; Brain; Hippocampus; |
Platform | MS-based; LC-MS: Waters AcquityTM UPLC system combined with the use of Waters XevoTM Q-TOF mass analyzer (Waters Corporation, Milford, MA, USA), Ultimate 3000 (Dionex, Sunnyvale, CA, USA) coupled to a Thermo Orbitrap Elite with a heated electrospray ion source (HESI-II) (Thermo Fisher Scientifc, Waltham, MA, USA); |
PMID | |
DOI | |
Citation | Geng C, Qiao Y, Guo Y, et al. Integrated metabolomics and lipidomics profiling of hippocampus reveal metabolite biomarkers in a rat model of chronic unpredictable mild stress-induced depression. Ann Transl Med. 2019;7(23):781. |
Metabolite | Gamma-Aminobutyric acid; L-Tryptophan; L-Threonine; L-Methionine; L-Glutamine; D-Glucose; L-Arginine; LysoPC(16:0); Kynurenic acid; Phenylacetylglycine; Xanthine; Indole-3-carboxylic acid; Glucose 6-phosphate; L-Histidine; Histamine; LysoPC(18:0); Betaine aldehyde; Glycerophosphocholine; L-Carnitine; PC(38:5); Purine; Phenylacetic acid; LysoPE(18:2); LysoPE(18:0); PC(32:0); Cinnamic acid; N-Acetylleucine; Proline betaine; LysoPE(20:0); Mannitol; PC(30:0); PC(36:1); LysoPE(18:1p); LysoPE(18:0p); PS(36:1); PS(38:4); PS(38:3); PE(36:3); PS(36:2); PS(40:6); PS(40:4); PC(34:1); LysoPI(18:0); Acetylcholine; LysoPC(22:5); LysoPC(20:3); LysoPC(24:0/0:0); Ketoleucine; D-Xylulose; Tetradecenoic acid; Kynuramine; (S)-2-Propylpiperidine; Cer(d15:0/28:1); Cer(d18:1/14:0); Cer(d18:1/18:2); Cer(d18:1/24:2); Cer(d18:2/16:0); Cer(d34:0); CL(18:1/16:0/18:1/20:0); CL(20:4/18:0/18:0/18:0); DG(34:1e); DG(36:2p); 12-[5]-Ladderane-dodecanoic acid; LysoPC(16:0e); LysoPC(16:0p); LysoPC(18:0p); LysoPC(18:1p); LysoPC(18:2p); LysoPC(19:0); LysoPC(19:1); LysoPC(19:3); LysoPC(21:3); LysoPC(21:4); LysoPC(22:1); LysoPC(22:3); LysoPC(23:1); LysoPC(23:4); LysoPC(24:6); LysoPC(25:0); LysoPC(26:1); LysoPC(26:3); LysoPC(28:1); LysoPC(28:4); LysoPE(17:0); LysoPE(18:0e); LysoPE(19:0); LysoPE(20:0p); LysoPE(20:1p); LysoPE(20:5); LysoPE(22:0); LysoPE(24:1); LysoPG(16:0); LysoPG(18:1); LysoPG(20:3); LysoPG(20:4); LysoPG(22:4); LysoPG(22:5); LysoPG(22:6); LysoPI(16:0); LysoPI(18:1); LysoPI(20:4); LysoPS(18:1); LysoPS(22:4); LysoPS(22:6); MGMG(16:0); MGMG(18:1); OAHFA(42:4); OAHFA(42:8); OAHFA(46:10); PA(34:1); PA(34:2e); PA(36:1); PA(43:7); PC(16:0/20:3); |
