Study M208
Study name | Zhao HX 2018 |
Title | Integrative proteomics-metabolomics strategy for pathological mechanism of vascular depression mouse model |
Overall design | The aim of this study was to establish a protein metabolism regulatory network in a murine vascular depression (VD) model to elucidate a more comprehensive impact of VD on organisms based on the combination of LC-MS-based proteomics and metabolomics. Mice were divided into the following 2 groups: (1) control group (sham operation, n = 26), (2) GCI model group (ischemia-reperfusion injury, n = 31). The mice were kept for 3 days to heal the wounds before participating in the behavior tests. Among the whole sample of each group, the hippocampus of 6 mice were used for metabolomics analysis and 10 for metabolites targeted validation. |
Study Type | Type1; |
Data available | Unavailable |
Organism | Mouse; ICR mouse; |
Categories of depression | Animal model; Other animal model; Other animal model; |
Criteria for depression | Forced swimming test, tail suspension test |
Sample size | 30 |
Tissue | Central; Brain; Hippocampus; |
Platform | MS-based; LC-MS: Micro LC (Eksigent Micro LC200 Plus) and Triple Quad 6500 (AB SCIEX, Foster City, CA, USA), plus the tandem of Agilent 1290 Infinity Ultra High Performance Liquid Chromatograph and Agilent 6470 Triple Quad LC/MS (Agilent Technology, USA); MS-based; LC-MS: Agilent 1290 Infinity liquid chromatography system with Agilent 6538 Accurate Mass Quadrupole Time-of-Flight mass spectrometer (Agilent, USA); |
PMID | |
DOI | |
Citation | Zhao H, Du H, Liu M, et al. Integrative proteomics-metabolomics strategy for pathological mechanism of vascular depression mouse model. J Proteome Res 2018;17(1):656-69. |
Metabolite | Citric acid; Pyroglutamic acid; Oleic acid; N-Acetyl-L-aspartic acid; L-Phenylalanine; L-Tryptophan; L-Valine; L-Leucine; L-Glutamic acid; Arachidonic acid; L-Methionine; L-Glutamine; L-Aspartic acid; L-Tyrosine; L-Arginine; Uridine; Pantothenic acid; Hypoxanthine; Xanthine; Taurine; Creatine; Niacinamide; Inosine; L-Malic acid; Adenosine monophosphate; Maleic acid; Pyrophosphate; Phosphorylcholine; Oxidized glutathione; Glycerophosphocholine; Inosinic acid; Cytidine; dGDP; Palmitic amide; Tetradecanoylcarnitine; O-Phosphoethanolamine; L-Palmitoylcarnitine; Palmitoylethanolamide; Guanosine diphosphate mannose; N-Acetylaspartylglutamic acid; |
