Study M104
Study name | Du HL 2017a |
Title | Metabolic profiles revealed synergistically antidepressant effects of lilies and Rhizoma Anemarrhenae in a rat model of depression |
Overall design | In this study, untargeted metabolomics was applied using mass spectrometry to identify metabolic signatures associated with depression in serum and explored the antidepressant effects of lilies and Rhizoma Anemarrhenae on an experimental model of chronic unpredictable mild stress (CUMS). Meanwhile metabolomics based on UHPLC-Q-TOF-MS was used to study the change in metabolites in CUMS rat serum and to evaluate the effects of Rhizoma Anemarrhenae and lilies (alone and in combination). Rats were divided into 5 groups (n = 12 in each group): (1) control group, (2) CUMS group, (3) CUMS + fluoxetine group (stress plus fluoxetine treatment at the dose of 10 mg/kg), (4) CUMS + BH group (stress plus lily saponin treatment at the dose of 48 mg/kg), and (5) CUMS + ZB group (stress plus Rhizoma Anemarrhenae saponin and lily saponin 1:1 treatment at the dose of 48 mg/kg). Twelve samples per group (except for CUMS + fluoxetine group, with n = 11) were used for metabonomic analysis. The CUMS stress procedure lasted for 6 weeks, and drugs were administered by gastric gavage once daily during the model building period. |
Study Type | Type1; Type2; |
Data available | Unavailable |
Organism | Rat; Sprague-Dawley rat; |
Categories of depression | Animal model; Chronic mild stress model; Chronic mild stress model; |
Criteria for depression | Sucrose preference test, forced swimming test |
Sample size | 59 |
Tissue | Peripheral; Blood; Serum; |
Platform | MS-based; LC-MS: Agilent 1290 Infinity LC system equipped with Agilent 6538 accurate mass quadrupole time-of-flight mass spectrometer (Agilent, USA); |
PMID | |
DOI | |
Citation | Du H, Zhao H, Lai X, et al. Metabolic profiles revealed synergistically antidepressant effects of lilies and Rhizoma Anemarrhenae in a rat model of depression. Biomed Chromatogr 2017;31(7). |
Metabolite | Citric acid; L-Phenylalanine; L-Tryptophan; L-Valine; L-Proline; L-Glutamic acid; Arachidonic acid; L-Methionine; Indole-3-propionic acid; Hippuric acid; Cholic acid; Propionylcarnitine; Butyrylcarnitine; Phenylacetylglycine; Oxoglutaric acid; Hydroxyphenyllactic acid; Serotonin; Betaine; Indolelactic acid; L-Carnitine; Indoleacrylic acid; Oleamide; Allantoin; Palmitic amide; |
