Study M096

Study name

Yu XY 2016

Title

A metabolomics-based approach for ranking the depressive level in a chronic unpredictable mild stress rat model

Overall design

In this study, an untargeted metabolomics study to investigate the metabolome change in plasma, hippocampus and prefrontal cortex (PFC) in an animal model with a major depressive disorder (MDD) had been conducted. Metabolomic profiling for the different bio-samples was analyzed by using Ultra-High Performance Liquid Chromatography coupled with Orbitrap mass spectrometry (UPLC-Orbitrap-MS). Rats were divided into 3 groups (n = 10 in each group): (1) control group, (2) CUMS group, and (3) CUMS + paroxetine group (stress plus paroxetine treatment at the dosage of 10 mg/kg). The CUMS stress procedure lasted for 7 weeks, and paroxetine was administered intraperitoneally once a day during the last 4 weeks of the model building period.

Study Type

Type1;

Type2;

Data available

Unavailable

Organism

Rat; Wistar rat;

Categories of depression

Animal model; Chronic mild stress model; Chronic mild stress model;

Criteria for depression

Sucrose preference test, forced swimming test

Sample size

30

Tissue

Peripheral; Blood; Plasma;

Central; Brain; Hippocampus;

Central; Brain; Prefrontal cortex;

Platform

MS-based; LC-MS: UPLC Ultimate 3000 system (Dionex, Germering, Germany) with Orbitrap mass spectrometer (Thermo Fisher Scientific, Bremen, Germany);

PMID

N/A

DOI

10.1039/C6RA00665E

Citation

Yu X, Qiao S, Wang D, et al. A metabolomics-based approach for ranking the depressive level in a chronic unpredictable mild stress rat model. RSC Adv 2016;6,25751-65.

Metabolite

myo-Inositol;

Gamma-Aminobutyric acid;

Citric acid;

Glycerol;

N-Acetyl-L-aspartic acid;

L-Phenylalanine;

L-Tryptophan;

L-Valine;

Glycine;

L-Serine;

L-Isoleucine;

L-Aspartic acid;

L-Lysine;

Homovanillic acid;

Tyramine;

Succinic acid;

L-Tyrosine;

5-Hydroxyindoleacetic acid;

D-Glucose;

Azelaic acid;

L-Kynurenine;

LysoPC(16:0);

Kynurenic acid;

Hypoxanthine;

DOPA;

Docosahexaenoic acid;

Beta-Alanine;

Inosine;

Dopamine;

Dihydroxyacetone phosphate;

Sorbitol;

Quinolinic acid;

3-Hydroxyhexadecanoic acid;

Glutathione;

LysoPE(16:0);

LysoPE(18:0);

Corticosterone;

LysoPC(20:4);

PC(14:0);

LysoPE(20:0);

LysoPC(20:5);

LysoPC(22:6);

Homocysteine;

Alpha-Linolenic acid;

PC(16:0);