Study M033
Study name | Webhofer C 2013 |
Title | Proteomic and metabolomic profiling reveals time-dependent changes in hippocampal metabolism upon paroxetine treatment and biomarker candidates |
Overall design | The aim of this study was to identify hippocampal cellular pathway alterations in DBA/2 mice using paroxetine as a representative selective serotonin reuptake inhibitor (SSRI). Furthermore, biomarker candidates for the assessment of antidepressant treatment effects in plasma were identified. The time course of metabolite level changes in hippocampus and plasma were studied using a targeted polar metabolomics profiling platform. Mice were divided into 2 groups (n = 6 in each group): (1) control group and (2) paroxetine group. Mice were treated twice per day with paroxetine (10 mg/kg) or vehicle (tap water) by gavaging for 4 weeks. Five samples per group were used for metabonomic analysis. |
Study Type | Type3; |
Data available | http://www.sciencedirect.com/science/article/pii/S0022395612003457 |
Organism | Mouse; DBA/2OlaHsd mouse; |
Categories of depression | Healthy individuals; Healthy individuals; Healthy individuals; |
Criteria for depression | Not reported |
Sample size | 10 |
Tissue | Central; Brain; Hippocampus; Peripheral; Blood; Plasma; |
Platform | MS-based; LC-MS: Prominence UFLC HPLC system (Shimadzu, Columbia, MD, USA) with 5500 QTRAP triple quadrupole mass spectrometer (AB/SCIEX, Framingham, MA, USA); |
PMID | |
DOI | |
Citation | Webhofer C, Gormanns P, Reckow S, et al. Proteomic and metabolomic profiling reveals time-dependent changes in hippocampal metabolism upon paroxetine treatment and biomarker candidates. J Psychiatr Res 2013;47(3):289-98. |
Metabolite | myo-Inositol; Gamma-Aminobutyric acid; Citric acid; L-Cysteine; L-Tryptophan; L-Valine; L-Serine; L-Threonine; L-Methionine; L-Alanine; L-Glutamine; Succinic acid; L-Tyrosine; L-Cystine; Oxoglutaric acid; Ascorbic acid; Indole-3-carboxylic acid; Fumaric acid; Fructose 6-phosphate; Glucose 6-phosphate; Taurine; Uracil; Betaine; Leucine or Isoleucine; Inosine; Citrulline; Argininosuccinic acid; CDP-Ethanolamine; Hexose-phosphate; 5-Thymidylic acid; dGTP; Adenosine triphosphate; Guanosine triphosphate; Cytidine triphosphate; Dihydroxyacetone phosphate; SBP; Uridine triphosphate; Glucose 1-phosphate; 3-Phosphoglyceric acid; Fructose 1,6-bisphosphate; Glyceraldehyde 3-phosphate; NAD; Biotin; Deoxyadenosine monophosphate; DL-2-Aminooctanoic acid; Hydroxyisocaproic acid; Orotidylic acid; 2'-Deoxyguanosine 5'-monophosphate; ADP-glucose; Thiamine monophosphate; 2-Keto-D-gluconic acid; 5'-Methylthioadenosine; L-Malic acid; Adenosine; NADP; Flavone; Guanosine monophosphate; Allantoic acid; L-Acetylcarnitine; Methylmalonic acid; Nicotinamide ribotide; Taurodeoxycholic acid; Phenylpropiolic acid; S-Adenosylhomocysteine; Adenosine monophosphate; Betaine aldehyde; Maleic acid; Pyrophosphate; Phosphorylcholine; Oxidized glutathione; Thiamine pyrophosphate; Alpha-ketoisovaleric acid; 2-Ketohexanoic acid; Deoxyadenosine; 1-Methylnicotinamide; Glycerophosphocholine; D-Ribose 5-phosphate; CDP; Carbamoyl phosphate; S-Ribosyl-L-homocysteine; 2-Isopropylmalic acid; Aminoadipic acid; Inosinic acid; 2-Hydroxyglutarate; N-Acetyl-L-alanine; Ureidosuccinic acid; |
