Study M691
Study name | Li X 2021 |
Title | Integrated network pharmacology and metabolomics to dissect the combination mechanisms of Bupleurum chinense DC-Paeonia lactiflora Pall herb pair for treating depression |
Overall design | In this study, the combined effect of Chaihu-Baishao was evaluated by the chronic unpredictable mild stress (CUMS) rat model. Sprague-Dawley rats were divided into the following 5 groups (n = 8 in each group): (1) control group, (2) CUMS group, (3) CUMS + low dose of Baishao group (stressor plus Baishao treatment at the dose of 7.5 g/kg), (4) CUMS + low dose of Chaihu group (stressor plus Chaihu treatment at the dose of 7.5 g/kg), and (5) CUMS + low dose of Chaihu-Baishao group (stressor plus Chaihu-Baishao treatment at the dose of 7.5 g/kg). The CUMS stress procedure lasted for 4 weeks, and drugs were administered via intragastric once per day during the model building period. Plasma metabolomics based on ultra-high performance liquid chromatography combined with quadrupole time-of-flight mass spectrometry (UPLC-QTOF/MS) was performed to discover the collaborative effect on metabolite regulation. |
Study Type | Type1; Type2; |
Data available | Unavailable |
Organism | Rat; Sprague-Dawley rat; |
Categories of depression | Animal model; Chronic mild stress model; Chronic mild stress model; |
Criteria for depression | Sucrose preference test, forced swimming test |
Sample size | 40 |
Tissue | Peripheral; Blood; Plasma; |
Platform | MS-based; LC-MS: Thermo-Fisher Dionex UltiMate 3000 UHPLC-Q Exactive Orbitrap-MS (Thermo Fisher, USA); |
PMID | |
DOI | |
Citation | Li X, Qin XM, Tian JS, et al. Integrated network pharmacology and metabolomics to dissect the combination mechanisms of Bupleurum chinense DC-Paeonia lactiflora Pall herb pair for treating depression. J Ethnopharmacol. 2021;264:113281. |
Metabolite | Pyroglutamic acid; Stearic acid; L-Phenylalanine; L-Tryptophan; L-Valine; Arachidonic acid; L-Arginine; Cholic acid; Phenylacetylglycine; L-Acetylcarnitine; Oleamide; Pipecolic acid; Deoxycholic acid; Spermidine; Corticosterone; L-Palmitoylcarnitine; Sphinganine; |
