Study M542
Study name | Han XM 2019 |
Title | Development of an underivatized LC-MS/MS method for quantitation of 14 neurotransmitters in rat hippocampus, plasma and urine: application to CUMS induced depression rats |
Overall design | This study developed an underivatized liquid chromatography tandem mass spectrometry (LC-MS/MS) method for simultaneous monitoring the 3 components of glutamate metabolism in rat hippocampus and 11 components of tryptophan and phenylalanine metabolism in rat hippocampus, plasma and urine, and applied it to investigate their changes in rats induced by chronic unpredictable mild stress (CUMS). Sprague-Dawley rats were divided into the following 2 groups (n = 8 in each group): (1) control group, and (2) CUMS group. The CUMS stress procedure lasted for 4 weeks. |
Study Type | Type1; |
Data available | Unavailable |
Organism | Rat; Sprague-Dawley rat; |
Categories of depression | Animal model; Chronic mild stress model; Chronic mild stress model; |
Criteria for depression | Sucrose preference test |
Sample size | 16 |
Tissue | Central; Brain; Hippocampus; Peripheral; Blood; Plasma; Peripheral; Urine; Urine; |
Platform | MS-based; LC-MS: Agilent 1200 series HPLC (Agilent, Santa Clara, CA, USA) with AB Sciex Qtrap 4000 tandem mass spectrometer (Foster City, CA, USA); |
PMID | |
DOI | |
Citation | Han XM, Qin YJ, Zhu Y, et al. Development of an underivatized LC-MS/MS method for quantitation of 14 neurotransmitters in rat hippocampus, plasma and urine: application to CUMS induced depression rats. J Pharm Biomed Anal. 2019;174:683-695. |
Metabolite | Gamma-Aminobutyric acid; L-Tryptophan; L-Tyrosine; L-Kynurenine; Xanthurenic acid; Serotonin; 5-HIAA/5-HT ratio; |
