Study M101
Study name | Bharwani A 2017 |
Title | Oral treatment with Lactobacillus rhamnosus attenuates behavioural deficits and immune changes in chronic social stress |
Overall design | Male C57BL/6 mice treated orally over 28 days with either Lactobacillus rhamnosus (JB-1) or vehicle were subjected to chronic social defeat (CSD) and assessed for alterations in behaviour and immune cell phenotype. 16S rRNA sequencing and LC-MS were employed to analyse the faecal microbial community and metabolite profile. Mice were divided into 4 groups: (1) control group (no stress and no drug, n = 10), (2) CSD group (stress and no drug, n = 10), (3) JB-1 group (no stress and JB-1 treatment, n = 5), and (4) CSD + JB-1 group (stress plus JB-1 treatment, n = 10). JB-1 treatment was gavaged at the dose of 200 ul (1.67 x 109 CFU). Treatment was administered over 28 days. During the final 10 days of treatment, chronic social defeat (CSD) was initiated. |
Study Type | Type1; Type2; |
Data available | Unavailable |
Organism | Mouse; C57BL/6 mouse; |
Categories of depression | Animal model; Social defeat model; Social defeat model; |
Criteria for depression | Social interaction test |
Sample size | 35 |
Tissue | Peripheral; Faece; Faece; |
Platform | MS-based; LC-MS: Waters ACQUITY ultra-performance liquid chromatography (UPLC) with Thermo Scientific Q-Exactive high resolution/accurate mass spectrometer; |
PMID | |
DOI | |
Citation | Bharwani A, Mian MF, Surette MG, et al. Oral treatment with Lactobacillus rhamnosus attenuates behavioural deficits and immune changes in chronic social stress. BMC Med 2017;15(1):7. |
Metabolite | Glyceric acid; Glutaric acid; Hippuric acid; Tyramine; 5-Hydroxyindoleacetic acid; L-Kynurenine; Pyroglutamine; Urea; Phenylacetylglycine; Xanthurenic acid; Indoxyl sulfate; Trimethylamine N-oxide; 4-Hydroxybutyric acid; Gluconic acid; 3-Hydroxy-3-(3-hydroxyphenyl)propanoic acid-O-sulphate; 1-Methylhistidine; N-a-Acetylcitrulline; 1-Methylnicotinamide; Glycerophosphocholine; L-Carnitine; Quinolinic acid; Allantoin; p-Cresol sulfate; Hexanoylcarnitine; Deoxycholic acid; Lithocholic acid; Guanidoacetic acid; Lactobionate; SM(d38:1); Tauro-b-muricholic acid; SM(d42:2); PE(18:0/18:2); SM(d18:1/22:0); SM(d18:1/18:0); N-Formyl-dl-phenylalanine; 2-Stearoyl-glycerophosphoethanolamine; PC(16:0/18:0); 1-Linolenoylglycerol; MG(0:0/16:0/0:0); 1-Docosahexaenoylglycerol; 4-Hydroxycinnamic acid; (R)-10-Hydroxystearate; 3-Hydroxyisovalerylcarnitine; Alpha-Muricholic acid; Hyodeoxycholic acid; N2,N6-diacetyllysine; (R)-3-hydroxybutyrylcarnitine; 1-(3-aminopropyl)-2-pyrrolidone; Cholate sulfate; Ortho-Hydroxyphenylacetic acid; Acetylcysteine; Equol sulfate; 2-Aminophenol sulphate; Guanidinosuccinic acid; S-(3-Hydroxypropyl)mercapturic Acid; 4-Methylcatechol sulfate; Ureidopropionic acid; Gulonic acid; Valerylglycine; Salicyluric acid; Propionylglycine; Phenol sulphate; Catechol Sulfate; 4-Ethylphenylsulfate; |
