Study M079
Study name | Guo YJ 2016 |
Title | Quantitative profiling of neurotransmitter abnormalities in the hippocampus of rats treated with lipopolysaccharide: focusing on kynurenine pathway and implications for depression |
Overall design | In this study, the effect of an intraperitoneal LPS injection (3 mg/kg) on the concentrations of neurotransmitters was investigated by in vivo microdialysis in rat hippocampus. To further explore dysregulation pattern of the neurotransmitters following continuous inflammatory process, the neurotransmitters in the hippocampus of rats after were analyzed 2-week LPS exposure (500 ug/kg every other day). Neurotransmitters and their metabolites in the hippocampus were determined by using high-performance liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS). In Experiment 1, the rats were divided into 2 groups (n = 7 in each group): (1) control group, (2) acute LPS group (3 mg/kg single dose of LPS intraperitoneally). In Experiment 2, the rats were divided into 2 groups (n = 7 in each group): (1) control group, (2) prolonged LPS group (500 ug/kg of LPS intraperitoneally every other day for 2 weeks). |
Study Type | Type1; |
Data available | Unavailable |
Organism | Rat; Sprague-Dawley rat; |
Categories of depression | Animal model; Lipopolysaccharide induced depression model; Lipopolysaccharide induced depression model; |
Criteria for depression | Sucrose preference test, forced swimming test |
Sample size | 28 |
Tissue | Central; Brain; Hippocampus; |
Platform | MS-based; LC-MS: Waters Acquity ultra-performance liquid chromatography system with Micromass Quattro Premier XE tandem quadruple mass spectrometer (Waters, USA); |
PMID | |
DOI | |
Citation | Guo Y, Cai H, Chen L, et al. Quantitative profiling of neurotransmitter abnormalities in the hippocampus of rats treated with lipopolysaccharide: focusing on kynurenine pathway and implications for depression. J Neuroimmunol 2016;295-296:41-6. |
Metabolite | Gamma-Aminobutyric acid; L-Tryptophan; L-Glutamic acid; Homovanillic acid; 5-Hydroxyindoleacetic acid; L-Kynurenine; Kynurenic acid; 3,4-Dihydroxybenzeneacetic acid; Serotonin; |
