Study M953

Study name

Li JN 2022

Title

Shugan granule contributes to the improvement of depression-like behaviors in chronic restraint stress-stimulated rats by altering gut microbiota

Overall design

The aim of this study was to evaluate the potential effect of Shugan Granule on the gut, brain, and behaviors in rats exposed to chronic restraint stress (CRS). Sprague-Dawley rats were divided into the following 3 groups (n = 8 in each group): (1) control group, (2) CRS group, and (3) CRS + Shugan Granule group (stressor plus Shugan Granule treatment at the dose of 0.63 g/kg). The CRS stress procedure lasted for 4 weeks, and drugs were administered via intragastric once per day during the model building period. Fecal samples were collected for GC-MS analysis.

Study Type

Type1;

Type2;

Data available

Unavailable

Organism

Rat; Sprague-Dawley rat;

Categories of depression

Animal model; Chronic restraint stress model; Chronic restraint stress model;

Criteria for depression

Sucrose preference test, forced swimming test

Sample size

24

Tissue

Peripheral; Faece; Faece;

Platform

MS-based; GC-MS: Agilent 7890B gas chromatography system with Agilent 5977A MSD system (Agilent, USA);

PMID

35713215

DOI

10.1111/cns.13881

Citation

Li J, Li Y, Duan W, et al. Shugan granule contributes to the improvement of depression-like behaviors in chronic restraint stress-stimulated rats by altering gut microbiota. CNS Neurosci Ther. 2022 Sep;28(9):1409-1424.

Metabolite

Citric acid;

Glycerol;

Pyroglutamic acid;

Palmitic acid;

L-Cysteine;

L-Tryptophan;

L-Leucine;

L-Glutamic acid;

L-Methionine;

L-Asparagine;

L-Aspartic acid;

5-Hydroxyindoleacetic acid;

Uridine;

Pseudouridine;

Pantothenic acid;

Threonic acid;

Chenodeoxycholic acid;

Phytosphingosine;

Creatinine;

Hypoxanthine;

Uric acid;

Ascorbic acid;

Xanthine;

Uracil;

Pyruvic acid;

5-Methoxytryptamine;

L-Malic acid;

Alpha-ketoisovaleric acid;

Inosinic acid;

3-Aminoisobutanoic acid;

4-Deoxyerythronic acid;

Malonic acid;

p-Hydroxyphenylacetic acid;

3-Hydroxyphenylacetic acid;

3,4-Dihydroxymandelic acid;

Alpha-Tocopherol;

Erythronic acid;

Dehydroascorbic acid;

5-Aminopentanoic acid;

Ribitol;

Aminomalonic acid;

Putrescine;

Cytosine;

Pipecolic acid;

L-Cystathionine;

Thymidine;

Thymine;

Deoxyguanosine;

Deoxycholic acid;

DL-O-Phosphoserine;

Benzoic acid;

Arabinofuranose;

Guanosine;

Rhamnose;

Guanidoacetic acid;

Maleimide;

Ribonic acid;

L-Arabitol;

5-Methoxyindoleacetate;

Mannitol;

Glycylproline;

Beta-mannosylglycerate;

Phytanic acid;

Gallic acid;

Gentisic acid;

3-Hydroxymethylglutaric acid;

Galactinol;

Dodecanoic acid;

Protocatechuic acid;

3,4-Dihydroxyhydrocinnamic acid;

Caffeic acid;

3-Hydroxybenzoic acid;

Pelargonic acid;

Glucaric acid;

2-Indolecarboxylic acid;

N-Methyl-L-glutamic acid;

Pentadecanoic acid;

4-Hydroxyhippuric acid;

Tryptamine;

Pectin;

N2-Acetylornithine;

Glutamylvaline;

Deoxyribose;

Conduritol-beta-epoxide;

Daidzein;

Palatinitol;

2-Piperidinobenzonitrile;

p-Octopamine;

Stigmasterol;

Tromethamine;

Chlorogenic acid;

Nonadecanoic acid;

D-myo-Inositol 4-phosphate;

N-Acetylmannosamine;

3-Palmitoyl-sn-glycerol;

5-Hydroxyindole-2-carboxylic acid;

Saccharopine;

6-hydroxy-2-aminohexanoic acid;

Triphenyl-phosphine imide;

Acifluorfen;

Normorphine;

Estradiol;

2-desoxy-pentos-3-ulose;

Fructose 1-phosphate;

Putreanine;

Bumetanide;

2,3-Dihydroxy-2-butenedioic acid;

Butorphanol;

Xylofuranose;

3,5-Dihydroxybenzoic acid;

Resveratrol;

alpha-Tocopherol acetate;

5-Nonanol;

2,4-diaminobutyric acid;

3-methoxy-5-prop-2-en-1-yl-2-hydroxy-n-2-hydroxyethylbenzamide;

Isomaltose;

Methylphosphonothiolate;

Naphthalene-1,8-diol;

Pimaric acid;

N-Acetyl-D-tryptophan;

Boric acid (H3BO3);

2-aminoheptanedioic acid;

Acebutolol;

2,3-Dihydroxy-acrylic acid;

Galacturonic acid;

cyclo(gly-gly);

Dimethyluric acid;

Isoproterenol;

1,4-dihydroxy-2,6-dimethoxybenzene;

Sophorose;

4-Hydroxyamphetamine;

3,3',4'5-Tetrahydroxystilbene;

Mannofuranuronic acid;

2-O-Methylascorbic acid;

Melibiose;

Catechollactate;

DL-Dopa;

Ergosterol;

Maltitol;

p-Hydroxymandelic acid;

3-methylene-2-phenyl-1-hydroxy-cyclopentene;

Coniferin;

Hexuronic acid;

Methyl beta-D-glucopyranoside;

2,3-Dihydro-8-methoxyfuro(2,3-b)quinoline;

2-Methyl-propanetriol;

5alpha-Cholestanol;

3-Aminopentanedioic acid;

2-Hydroxy-3-methylbutyric acid;

Ethyl beta-d-galactofuranoside;

Glycerol-3-galactoside;

3-Deoxy-d-mannitol;

Cyclohexanecarboxylic acid;

D-xylonic acid;

5-ethyl-1h-indole-2-carboxylic acid;

2-Deoxyerythritol;

1-Triacontanol;

Tyrosol;