Study name | Peric I 2022 |
Title | Metabolomic profiling relates tianeptine effectiveness with hippocampal GABA, myo-inositol, cholesterol, and fatty acid metabolism restoration in socially isolated rats |
Overall design | In this study, the comprehensive two-dimensional gas chromatography-mass spectrometry (GC x GC-MS)-based comparative non-targeted analysis was employed on hippocampus of rats to examine the effects of chronic social isolation (6 weeks) and/or chronic tianeptine treatment (10 mg/kg per day) in controls and chronic social isolation to obtain real-time metabolomic fingerprints representative of the underlying molecular changes. Wistar rats were divided into the following 5 groups: (1) control group (no stress, with vehicle treatment), (2) tianeptine group (no stress, with tianeptine treatment at the dose of 10 mg/kg), (3) chronic social isolation group (social isolation, with vehicle treatment), (4) chronic social isolation + tianeptine group (social isolation plus tianeptine treatment at the dose of 10 mg/kg, rats responding effectively to tianeptine treatment), and (5) chronic social isolation + tianeptine resilient group (social isolation plus tianeptine treatment at the dose of 10 mg/kg, rats responding ineffectively to tianeptine treatment). The chronic social isolation procedure lasted for 6 weeks, and tianeptine was administered with intraperitoneal injections during the model building period. Hippocampal and serum samples were used for metabonomic analysis (n =5-6/ group). |
Study Type  | Type1;
Type2;
Type3;
Type4;
|
Data available | Unavailable |
Organism | Rat; Wistar rat;
|
Categories of depression | Animal model; Other animal model; Other animal model;
Healthy individuals; Healthy individuals; Healthy individuals;
|
Criteria for depression | Forced swimming test |
Sample size | 29 |
Tissue | Central; Brain; Hippocampus;
Peripheral; Blood; Serum;
|
Platform | MS-based; GC-MS: GCMS-QP2010 Ultra (Shimadzu, Kyoto, Japan) comprehensive GC × GC-quadrupole MS; |
PMID | 35776189 |
DOI | 10.1007/s00213-022-06180-y |
Citation | Peric I, Ljesevic M, Beskoski V, et al. Metabolomic profiling relates tianeptine effectiveness with hippocampal GABA, myo-inositol, cholesterol, and fatty acid metabolism restoration in socially isolated rats. Psychopharmacology. 2022 Jul 1. |
Metabolite | myo-Inositol;
Gamma-Aminobutyric acid;
Pyroglutamic acid;
Stearic acid;
Palmitic acid;
Glycine;
L-Threonine;
Arachidonic acid;
L-Aspartic acid;
Pantothenic acid;
Niacinamide;
Cholesterol;
alpha-D-Glucose;
Elaidic acid;
Deoxyadenosine;
D-Galactose;
11Z-Eicosenoic acid;
D-Arabitol;
1-Methyladenosine;
2-Monostearin;
1,5-Anhydrosorbitol;
10,12-Docosadiynedioic acid;
Methylphosphate;
Pentadecanoic acid;
Ribothymidine;
Palmitelaidic acid;
Stearic acid, 2-(1-octadecenyloxy)ethyl ester, (Z)-;
Ethyl iso-allocholate;
trans-13-octadecenoic acid;
Octadec-9-enoic Acid;
Hematoporphyrin;
6-Methyl-2-oxo-4-(3-trifuoromethyl-phenyl)-1,2,3,4-tetrahydro-pyrimidine-5-carboxylic acid 2-methylsulfanyl-ethyl ester;
D-Allofuranose;
Silane, (2-furanylmethoxy)trimethyl-;
D-Glucopyranose, 2-(acetylamino)-2-deoxy;
GABA, Butanoic acid, 4-[bis(trimethylsilyl)amino]-;
Trifluoroacetamide;
But-2-enoic acid;
Glycine, N-[(3alpha,5beta,7alpha,12alpha)-24-oxo-3,7,12-tris[(trimethylsilyl)oxy]cholan-24-yl]-, methyl ester;
Cholest-5-en-3-ol;
Cholestane-3,5-diol, 5-acetate, (3beta,5alpha)-;
Ketamine;
Xylazine;
Norketamine;
1-Methyl-N,N-bis(trimethylsilyl)-4-[(trimethylsilyl)oxy]-1H-imidazol-2-amine;
3-Oxaoct-4-en-2-imine;
alpha-D-Glucopyranosiduronic acid, 3-(5-ethylhexahydro-2,4,6-trioxo-5-pyrimidinyl)-1,1-dimethylpropyl, methyl ester;
Methyl(n-butyl)amine;
Citric acid, 1,2,3-propanetricarboxylic acid;
n-Heptadecan-1,2-diol;
Succinic acid, butanedioic acid;
beta-N-Acetylglucosamine;
beta-N-Acetylneuraminic acid;
Silanamine, N,N'-methanetetraylbis[1,1,1-trimethyl-;
Octadecanoic acid, 2,3-bis[(trimethylsilyl)oxy]propyl ester;
L-Asparagine, N,N2-bis(tert-butyldimethylsilyl)-, tert-butyldimethylsilyl ester;
2alpha,3beta-Diacetoxy-2-methoxy-2,3-dihydrobiphenylene;
Trifluoroacetamide, N-trimethylsilyloxymethyl-;
Tris(trimethylsilyl)carbamate;
D-Glucopyranose, 2-(acetylamino)-2-deoxy-1,3,4,6-tetrakis-O-(trimethylsilyl)-;
cis-6-Octadecenoic acid;
9-Octadecenoic acid, 2-[(trimethylsilyl)oxy]-1-[[(trimethylsilyl)oxy] methyl]ethyl ester;
Hexadecanoic acid, 2,3-bis[(trimethylsilyl)oxy]propyl ester;
Phosphoric acid, bis(trimethylsilyl)monomethyl ester;
2'-Deoxyadenosine, bis(trimethylsilyl) deriv.;
2beta,4alpha-Epoxymethylphenanthrene-7-methanol, 1,1-dimethyl-2-methoxy-8-(1,3-dithiin-2-ylidene)methyl-1,2,3,4,4a,4b,5,6,7,8,8a,9-dodecahydro-, acetate;
N-trimethylsilylaziridine;
alpha-D-Glucopyranoside, methyl 2-(acetylamino)-2-deoxy-3-O-(trimethylsilyl)-, cyclic methylboronate;
Hexadecanoic acid, 1-(hydroxymethyl)-1,2-ethanediyl ester;
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