Overall design | This exploratory study was a targeted metabolomic analysis carried out on the plasma and cerebrospinal fluid (CSF) of 9 healthy human participants who received a 40 min intravenous infusion of ketamine (0.5 mg/kg) to determine whether changes in the plasma corresponded to similar changes in the CSF. Healthy volunteers received a ketamine infusion (0.5 mg/kg/40 min IV) and contributed plasma and CSF samples for up to 28 h. Blood and CSF were collected at baseline, 40 min, 120 min, and 230 min, as well as at 6, 10, 12, 22, 24, 26, and 28 h post-infusion. Parallel blood draws with a 48 h timepoint were also collected for blood. A targeted metabolomic assessment was carried out, which quantifies 630 distinct metabolites in human and rodent plasma, human CSF, and mouse brain. |
Citation | Moaddel R, Zanos P, Farmer CA, et al. Comparative metabolomic analysis in plasma and cerebrospinal fluid of humans and in plasma and brain of mice following antidepressant-dose ketamine administration. Transl Psychiatry. 2022 May 2;12(1):179. |
