Study M869
Study name | Huang DH 2022a |
Title | Metabolomics based on peripheral blood mononuclear cells to dissect the mechanisms of Chaigui granules for treating depression |
Overall design | In this study, the chronic unpredictable mild stress (CUMS) rat model was used to evaluate the antidepressant effect of Chaigui granules. Sprague-Dawley rats were divided into the following 4 groups (n = 8 in each group): (1) control group, (2) CUMS group, (3) CUMS + middle dose of Chaigui granules group (stressor plus Chaigui granules treatment at the dose of 8.3 g/kg), and (4) CUMS + high dose of Chaigui granules group (stressor plus Chaigui granules treatment at the dose of 16.6 g/kg). The CUMS stress procedure lasted for 4 weeks, and drugs were administered via intragastric once per day during the model building period. Peripheral blood mononuclear cell (PBMC) metabolomics was used to search the key metabolites and metabolic pathways regulated by Chaigui granules. Six samples per group were used for metabonomic analysis. |
Study Type | Type1; Type2; |
Data available | Unavailable |
Organism | Rat; Sprague-Dawley rat; |
Categories of depression | Animal model; Chronic mild stress model; Chronic mild stress model; |
Criteria for depression | Sucrose preference test, forced swimming test |
Sample size | 24 |
Tissue | Peripheral; Blood; Peripheral blood mononuclear cell; |
Platform | MS-based; LC-MS: Thermo-Fisher Dionex UltiMate 3000 UHPLC-Q Exactive Orbitrap-MS; |
PMID | |
DOI | |
Citation | Huang D, Wang L, Wu Y, et al. Metabolomics based on peripheral blood mononuclear cells to dissect the mechanisms of Chaigui granules for treating depression. ACS Omega 2022. |
Metabolite | Palmitic acid; L-Tyrosine; Phytosphingosine; Hypoxanthine; Betaine; Adenosine monophosphate; L-Carnitine; Oleamide; Octadecanamide; Palmitic amide; Spermidine; Sphingosine 1-phosphate; Palmitoylethanolamide; 13-HODE; LysoPE(18:0/0:0); Docosapentaenoic acid (22n-3); Docosadienoate (22:2n6); MG(18:0/0:0/0:0); Docosatrienoic acid; |
