Study M867
Study name | Gu S 2022 |
Title | Develop a stepwise integrated method to screen biomarkers of Baihe-Dihuang Tang on the treatment of depression in rats applying with composition screened, untargeted and targeted metabolomics analysis |
Overall design | In this study, a comprehensive metabolomic analysis was used to explore for the potential mechanism of Baihe-Dihuang Tang in chronic unpredictable mild stress (CUMS)-induced depression in rats. Sprague-Dawley rats were divided into the following 4 groups (n = 7 in each group): (1) control group, (2) CUMS group, (3) CUMS + Baihe-Dihuang Tang group (stressor plus Baihe-Dihuang Tang treatment at the dose of 15 g/kg), and (4) CUMS + fluoxetine group (stressor plus fluoxetine treatment at the dose of 3.6 mg/kg). The CUMS stress procedure lasted for 6 weeks, and drugs were administered via intragastric once per day from the 22 day of the model building period. Untargeted metabolomics based on ultra-high performance liquid chromatography with quadrupole time-of-flight mass spectrometry was performed to detect variations in the metabolites/metabolite categories in the brain, plasma, and urine of rats. The contents of dopamine, norepinephrine, 5-hydroxytryptamine, L-glutamate, xanthine, and adenine in the brain were quantified by targeted ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) metabolomics. |
Study Type | Type1; Type2; |
Data available | Unavailable |
Organism | Rat; Sprague-Dawley rat; |
Categories of depression | Animal model; Chronic mild stress model; Chronic mild stress model; |
Criteria for depression | Sucrose preference test |
Sample size | 28 |
Tissue | Central; Brain; Brain; Peripheral; Blood; Plasma; Peripheral; Urine; Urine; |
Platform | MS-based; LC-MS: Masslynx-controlled Waters ACQUITY UPLC system with Xevo G2-XS QTOF-MS (Waters Corporation, Milford, MA, USA); MS-based; LC-MS: Agilent 1290 system (Agilent, CA, USA) with Triple Quad™ 3500 MS/MS system (Applied AB Sciex, Foster City, CA, USA); |
PMID | |
DOI | |
Citation | Gu S, Mou T, Chen J, et al. Develop a stepwise integrated method to screen biomarkers of Baihe-Dihuang Tang on the treatment of depression in rats applying with composition screened, untargeted and targeted metabolomics analysis. J Sep Sci. 2022 Mar 2. |
Metabolite | myo-Inositol; Pyroglutamic acid; Stearic acid; Palmitic acid; L-Proline; L-Glutamic acid; L-Tyrosine; Phenylpyruvic acid; Taurocholic acid; Cholic acid; Chenodeoxycholic acid; Phenylacetylglycine; Xanthine; DOPA; 5-Hydroxy-6-methoxyindole glucuronide; Inosine; Norepinephrine; 1-Methylhistidine; Indoleacetaldehyde; 2-Aminomuconic acid semialdehyde; N-Acetylserotonin; Deoxyadenosine monophosphate; Glycerophosphocholine; Adenine; D-Glucurono-6,3-lactone; Glycocholic acid; LysoPC(15:0/0:0); Pipecolic acid; Glutathione; D-Erythrose 4-phosphate; LysoPC(P-16:0); Taurochenodesoxycholic acid; (S)-3-Hydroxyisobutyric acid; Cortisol; D-Xylose; Arachidic acid; Sphingosine 1-phosphate; 5-Methoxyindoleacetate; Pyridoxamine; Deoxycytidine; LysoPC(20:0/0:0); Epinephrine; Tetrahydrocortisol; Estrone sulfate; Deoxycholic acid 3-glucuronide; LysoPC(O-18:0); Sphinganine; Sphinganine 1-phosphate; LysoPC(20:2(11Z,14Z)/0:0); Chenodeoxycholic acid glycine conjugate; 15-HETE; Retinyl ester; Ketoleucine; Dopaquinone; Homovanillin; 5-L-Glutamyl-taurine; 5-HETE; 6-Hydroxymelatonin; Formylanthranilic acid; Estrone glucuronide; LysoPC(P-18:1(9Z)); LysoPC(P-18:0/0:0); Pyrroline hydroxycarboxylic acid; 16a-Hydroxyestrone; 2-Aminobenzoic acid; 4-Guanidinobutanoic acid; 1-Pyrroline-5-carboxylic acid; 2-Hydroxyestrone; PC(22:0/P-18:1(11Z)); PC(24:0/24:1(15Z)); SN-38; 6-Dehydrotestosterone glucuronide; 3beta-Hydroxypregn-5-en-20-one sulfate; 3a,7a,12a-Trihydroxy-5b-cholestan-26-al; 3a,7a-Dihydroxy-5b-cholestan-26-al; 13-L-Hydroperoxylinoleic acid; 3a,6b,7a,12a-Tetrahydroxy-5b-cholanoic acid; 5b-Cholestane-3a,7a,12a,23R,25-pentol; 17alpha,21-Dihydroxypregnenolone; Dihydrocortisol; Riboflavin reduced; 6-Thiourate; 3beta-Hydroxy-5-cholestenoic acid; 2beta-Hydroxytestosterone; GlcCer(d18:1/22:0); Retinoyl b-glucuronide; Cytidine 2',3'-cyclic phosphate; 15-Hydroxynorandrostene-3,17-dione glucuronide; Octanoylglucuronide; 3-Carbamoyl-2-phenylpropionic acid; Acetyl-N-formyl-5-methoxykynurenamine; PC(22:0/P-18:1(9Z)); Galactosylceramide (d18:1/22:0); 6-Hydroxy-5-methoxyindole glucuronide; Nicotinamide riboside; |
