Study M850

Study name

Zhang R 2021b

Title

A reliable high-throughput screening model for antidepressant

Overall design

In this study, the authors established the chronic unpredictable mild stress (CUMS) model and reserpine model in mice and zebrafish, respectively. The effects of CUMS and reserpine were preliminarily analyzed through behavioral tests and brain pathology sections, and then compared metabolic differences through metabolomics. C57BL/6 mice were divided into the following 2 groups: (1) control group, and (2) reserpine group. Reserpine was injected intraperitoneally at a dose of 1 mg/kg for 3 days. Eight brain samples per group were used for metabonomic analysis.

Study Type

Type1;

Data available

Unavailable

Organism

Mouse; C57BL/6 mouse;

Categories of depression

Animal model; Other animal model; Other animal model;

Criteria for depression

Sucrose preference test, forced swimming test, tail suspension test

Sample size

16

Tissue

Central; Brain; Brain;

Platform

MS-based; LC-MS: Agilent 1260 high-performance liquid chromatography-quadrupole time-of-flight mass spectrometer (Agilent, Santa Clara, CA, USA);

PMID

34502414

DOI

10.3390/ijms22179505

Citation

Zhang R, Qiao C, Liu Q, et al. A reliable high-throughput screening model for antidepressant. Int J Mol Sci. 2021 Sep 1;22(17):9505.

Metabolite

L-Glutamic acid;

L-Glutamine;

L-Aspartic acid;

Tyramine;

Suberic acid;

5-Hydroxyindoleacetic acid;

Azelaic acid;

Uridine;

Xanthurenic acid;

Indoleacetic acid;

Hypoxanthine;

Glucose 6-phosphate;

Taurine;

Betaine;

Histamine;

Inosine;

Norepinephrine;

Citrulline;

5-Methoxytryptamine;

N-Acetylserotonin;

Ornithine;

Adenosine;

1-Methylnicotinamide;

L-Carnitine;

Adenine;

Melatonin;

5-Aminopentanoic acid;

Phosphoenolpyruvic acid;

Glutathione;

N-Acetylleucine;

Corticosterone;

2-Amino-3-phosphonopropionic acid;

Carnosine;

Oxalacetic acid;

Phosphocreatine;

Deoxycytidine;

5-Hydroxy-L-tryptophan;

Homocarnosine;

Dihomo-gamma-linolenic acid;

Deoxyuridine;

4-Hydroxybenzoic acid;

Indole;

5-Methylcytosine;

8-Hydroxy-deoxyguanosine;

Homo-L-arginine;

N-acetylhistamine;

Triethanolamine;

N6-Acetyl-L-lysine;

Phenobarbital;

4-Guanidinobutanoic acid;

Hexylamine;

Theobromine;

Isokobusone;

5-Methoxydimethyltryptamine;

Norspermidine;

Chalconaringenin;

Acetaminophen;

2-Methoxybenzoic acid;

N,N-Dimethylaniline;

6-Methylcoumarin;

Methomyl;

1,2,3,4-Tetrahydro-beta-carboline;

beta-Carboline;

Umbelliferone;

Glycylleucine;

Harmalan;

2-(Methylamino)benzoic acid;

Cotinine;

2(3H)-Benzothiazolethione;

(2-Naphthalenyloxy)acetic acid;

(E)-Monocrotophos;

4'-Methoxychalcone;

N-Desmethylvenlafaxine;

Norizalpinin;

Harmine;

Norfentanyl;

N,O-Didesmethylvenlafaxine;

Avocadyne;

Desvenlafaxine;

Parathion;

Cinchonidine;

Fexofenadine;

Ginkgolide A;

4-Methylbenzoic acid;

4-Methoxycinnamic acid;

4-Hydroxybenzaldehyde;

Indole-3-methyl acetate;

Cysteine-S-sulfate;

Zeatin;

Pinocembrin;