Study M847
Study name | Wang P 2021 |
Title | Dose-effect/toxicity of Bupleuri Radix on chronic unpredictable mild stress and normal rats based on liver metabolomics |
Overall design | In this study, the authors used the chronic unpredictable mild stress (CUMS) model to evaluate the effects and toxicities of Bupleuri Radix and to decipher the dose-effect/toxicity relationship and mechanism using the liver metabonomics combined with multivariate statistical analysis. Sprague-Dawley rats were divided into the following 3 groups (n = 8 in each group): (1) control group, (2) CUMS group, and (3) CUMS + Bupleuri Radix group (stressor plus Bupleuri Radix treatment at the dose of 50 g herb/kg). The CUMS stress procedure lasted for 3 weeks, and drugs were administered via intragastric once per day during the model building period. Liver samples were used for metabonomic analysis. |
Study Type | Type1; Type2; |
Data available | Unavailable |
Organism | Rat; Sprague-Dawley rat; |
Categories of depression | Animal model; Chronic mild stress model; Chronic mild stress model; |
Criteria for depression | Sucrose preference test |
Sample size | 24 |
Tissue | Peripheral; Liver; Liver; |
Platform | MS-based; LC-MS: Thermo-Fisher UHPLC with Q Exactive Orbitrap-MS (ThermoFisher, United States); |
PMID | |
DOI | |
Citation | Wang P, Gao X, Liang M, et al. Dose-effect/toxicity of Bupleuri Radix on chronic unpredictable mild stress and normal rats based on liver metabolomics. Front Pharmacol. 2021 Sep 7;12:627451. |
Metabolite | L-Valine; L-Leucine; L-Isoleucine; Succinic acid; L-Tyrosine; Phenylpyruvic acid; Phytosphingosine; Propionylcarnitine; Xanthine; Betaine; Niacinamide; Adenosine; L-Acetylcarnitine; Taurodeoxycholic acid; L-Carnitine; |
