Study M828

Study name

Abramova O 2021

Title

Brain metabolic profile after intranasal vs. intraperitoneal clomipramine treatment in rats with ultrasound model of depression

Overall design

The aims of this study were to clarify the variable frequency ultrasonic radiation impact on rats’ brain tissue, and to observe how tricyclic antidepressant clomipramine administrated in different ways affects metabolic processes in animal models and their behavior. Wistar rats were divided into the following 4 groups (n = 8 per group): (1) control group, (2) ultrasonic radiation group, (3) ultrasonic radiation + intranasally clomipramine group (stressor plus clomipramine treatment at the dose of 7.5 mg/kg, intranasally), and (4) ultrasonic radiation + intraperitoneally clomipramine group (stressor plus clomipramine treatment at the dose of 7.5 mg/kg, intraperitoneally). Rats in the ultrasonic radiation groups were exposed to chronic ultrasound stress for 3 weeks, and clomipramine was administered once per day during the model building period. Then 28 metabolites in the frontal cortex and hippocampus were analyzed by LC-MS.

Study Type

Type1;

Type2;

Data available

Unavailable

Organism

Rat; Wistar rat;

Categories of depression

Animal model; Other animal model; Other animal model;

Criteria for depression

Sucrose preference test, forced swim test, social interaction test

Sample size

32

Tissue

Central; Brain; Frontal cortex;

Central; Brain; Hippocampus;

Platform

MS-based; LC-MS: Shimadzu CBM-30ACMP liquid chromatograph with SCIEX 4500 QTRAP;

PMID

34502505

DOI

10.3390/ijms22179598

Citation

Abramova O, Zorkina Y, Syunyakov T, et al. Brain metabolic profile after intranasal vs. intraperitoneal clomipramine treatment in rats with ultrasound model of depression. Int J Mol Sci. 2021 Sep 4;22(17):9598.

Metabolite

Gamma-Aminobutyric acid;

L-Phenylalanine;

L-Tryptophan;

L-Glutamic acid;

Glycine;

L-Threonine;

L-Methionine;

L-Alanine;

L-Aspartic acid;

Uridine;

Hypoxanthine;

Xanthine;

Fructose 6-phosphate;

Niacinamide;

Inosine;

L-Acetylcarnitine;

Oxidized glutathione;

D-Ribose 5-phosphate;

L-Carnitine;

Adenine;

Guanosine;

Glyceraldehyde;