Study M823

Study name

Wang QS 2021a

Title

Targeting hippocampal phospholipid and tryptophan metabolism for antidepressant-like effects of albiflorin

Overall design

The objective of this study was to investigate the rapid antidepressant-like effects of albiflorin in the chronic unpredictable mild stress (CUMS) model of depression and elucidate the pharmaco-metabolic mechanisms of its action using a multi-omics approach. Sprague-Dawley rats were divided into the following 4 groups (n = 8 per group): (1) control group (non-stressor with vehicle treatment), (2) CUMS group (stressor with vehicle treatment), (3) CUMS + albiflorin group (stressor plus albiflorin treatment at the dose of 7 mg/kg), and (4) CUMS + fluoxetine group (stressor plus fluoxetine treatment at the dose of 10 mg/kg). The CUMS stress procedure lasted for 4 weeks, and drugs were administered via intragastric once per day for 1 week after stress. The changes in hippocampal metabolism were investigated using both metabolomic and lipidomic approaches.

Study Type

Type1;

Type2;

Data available

https://www.metabolomicsworkbench.org/data/DRCCMetadata.php?Mode=Study&StudyID=ST000903

Organism

Rat; Sprague-Dawley rat;

Categories of depression

Animal model; Chronic mild stress model; Chronic mild stress model;

Criteria for depression

Sucrose preference test

Sample size

32

Tissue

Central; Brain; Hippocampus;

Peripheral; Blood; Plasma;

Platform

MS-based; LC-MS: Waters ACQUITY UPLC with QTRAP 6500 MS/MS system (SCIEX, USA);

PMID

34601221

DOI

10.1016/j.phymed.2021.153735

Citation

Wang QS, Yan K, Li KD, et al. Targeting hippocampal phospholipid and tryptophan metabolism for antidepressant-like effects of albiflorin. Phytomedicine. 2021 Nov;92:153735.

Metabolite

N-Acetyl-L-aspartic acid;

L-Tryptophan;

L-Serine;

L-Cystine;

Dimethylglycine;

Serotonin;

Phosphatidylcholine;

Adenosine monophosphate;

L-Carnitine;

Quinolinic acid;

Nicotinic acid;

Melatonin;

Kynurenine/Tryptophan ratio;

Epinephrine;

Anandamide;

Oleoylethanolamide;

S-Adenosylmethionine;

20-Hydroxyeicosatetraenoic acid;

Phosphatidylethanolamine;

Phosphatidylinositol;

Phosphatidic acid;

Sphingomyelin;

Octadecadienylcarnitine;

12-HETE;

Ceramide;

Prostaglandin F2a;

Ethanolamine plasmalogens;

1-Pyrroline-5-carboxylic acid;