Study M767
Study name | Qu SY 2021 |
Title | Analysis of antidepressant activity of Huang-Lian Jie-Du Decoction through network pharmacology and metabolomics |
Overall design | The aim of this study was to explore antidepressant activity of Huang-Lian Jie-Du Decoction (HLJDD), and evaluating active components and potential depression-associated targets. HLJDD was administered on chronic unpredictable mild stress-induced (CUMS) depressive mice. C57BL/6 mice were divided into the following 3 groups (n = 10 in each group): (1) control group, (2) CUMS group, and (3) CUMS + HLJDD group (stressor plus HLJDD treatment at the dose of 590 mg/kg). The CUMS stress procedure lasted for 5 weeks, and drugs were administered via intragastric once per day during the last week of the model building period. Neurotransmitters including c-aminobutyric acid, glutamic acid, 5-hydroxytryptamine, dopamine and acetylcholine in the hippocampus, cortex, striatum and amygdala were determined using LC-MS/MS. |
Study Type | Type1; Type2; |
Data available | Unavailable |
Organism | Mouse; C57BL/6 mouse; |
Categories of depression | Animal model; Chronic mild stress model; Chronic mild stress model; |
Criteria for depression | Forced swimming test |
Sample size | 30 |
Tissue | Central; Brain; Striatum; Central; Brain; Amygdala; Central; Brain; Hippocampus; Central; Brain; Cortex; |
Platform | MS-based; LC-MS: LC-Q/TOF-MS (AB SCIEX Triple TOF 5600); MS-based; LC-MS: not reported; |
PMID | |
DOI | |
Citation | Qu SY, Li XY, Heng X, et al. Analysis of antidepressant activity of Huang-Lian Jie-Du Decoction through network pharmacology and metabolomics. Front Pharmacol. 2021 Mar 4;12:619288. |
Metabolite | Gamma-Aminobutyric acid; L-Tryptophan; L-Glutamic acid; 5-Hydroxyindoleacetic acid; L-Kynurenine; Creatinine; Serotonin; Hydroxykynurenine; Betaine; Dopamine; Glucose 1-phosphate; 3-Hydroxyanthranilic acid; Metanephrine; Picolinic acid; Acetylcholine; |
