Study M766
Study name | Ma SR 2021 |
Title | Determination and application of nineteen monoamines in the gut microbiota targeting phenylalanine, tryptophan, and glutamic acid metabolic pathways |
Overall design | In this study, the authors established an underivatized liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for the quantification of nineteen monoamine neurotransmitters and related metabolites in the gut microbiota. The neurotransmitters and related metabolites included phenylalanine, tyrosine, L-dopa, dopamine, 3-methoxytyramine, tryptophan, hydroxytryptophan, 5-hydroxytryptamine, 5-hydroxyindole-3-acetic acid, kynurenine, kynurenic acid, melatonin, tryptamine, indole-3-lactic acid, indole-3-acetic acid, indolyl-3-propionic acid, glutamic acid, gamma-aminobutyric acid, and acetylcholine. This method was validated in fresh fecal samples from chronic unpredictable stress (CUS) model of depression. Sprague-Dawley rats were divided into the following 2 groups (n = 6 in each group): (1) control group, and (2) CUS group. The CUS stress procedure lasted for 8 weeks. |
Study Type | Type1; |
Data available | Unavailable |
Organism | Rat; Sprague-Dawley rat; |
Categories of depression | Animal model; Chronic mild stress model; Chronic mild stress model; |
Criteria for depression | Sucrose preference test |
Sample size | 12 |
Tissue | Peripheral; Faece; Faece; |
Platform | MS-based; LC-MS: LC-MS/MS 8060 (Shimadzu Corporation, Kyoto, Japan); |
PMID | |
DOI | |
Citation | Ma SR, Yu JB, Fu J, et al. Determination and application of nineteen monoamines in the gut microbiota targeting phenylalanine, tryptophan, and glutamic acid metabolic pathways. Molecules. 2021 Mar 4;26(5):1377. |
Metabolite | L-Phenylalanine; L-Tryptophan; L-Glutamic acid; Indole-3-propionic acid; L-Tyrosine; 5-Hydroxyindoleacetic acid; L-Kynurenine; Kynurenic acid; |
