Study M632
Study name | Linghu T 2020 |
Title | A unique insight for energy metabolism disorders in depression based on chronic unpredictable mild stress rats using stable isotope-resolved metabolomics |
Overall design | In this study, the stable isotope-resolved metabolomics (SIRM) technology was applied to the chronic unpredictable mild stress (CUMS) model of depression. Sprague-Dawley rats were divided into the following 2 groups (n = 10 in each group): (1) control group, and (2) CUMS group. The CUMS stress procedure lasted for 4 weeks. Hydrophilic metabolites and hydrophobic metabolites were extracted through different solvents and separated on the reverse-phase column (T3) and the normal-phase column (HILIC). |
Study Type | Type1; |
Data available | Unavailable |
Organism | Rat; Sprague-Dawley rat; |
Categories of depression | Animal model; Chronic mild stress model; Chronic mild stress model; |
Criteria for depression | Sucrose preference test |
Sample size | 20 |
Tissue | Peripheral; Blood; Serum; |
Platform | MS-based; LC-MS: Thermo DIONEX UltiMate 3000 high performance liquid chromatograph with Thermo Q Exactive HF Hybrid Quadrupole-Orbitrap mass spectrometer (Thermo Fisher Scientific, Inc., Germany); |
PMID | |
DOI | |
Citation | Linghu T, Gao Y, Li A, et al. A unique insight for energy metabolism disorders in depression based on chronic unpredictable mild stress rats using stable isotope-resolved metabolomics. J Pharm Biomed Anal. 2020;191:113588. |
Metabolite | Glyceric acid; Gamma-Aminobutyric acid; L-Phenylalanine; L-Tryptophan; L-Leucine; L-Glutamic acid; L-Serine; L-Threonine; Arachidonic acid; L-Methionine; L-Lactic acid; L-Asparagine; L-Glutamine; D-Fructose; Uridine; Pantothenic acid; Taurocholic acid; Phenylacetylglycine; Pyruvic acid; Gluconic acid; Xanthosine; Citrulline; Ornithine; Phosphorylcholine; D-Galactose; LysoPC(20:4/0:0); LysoPE(0:0/18:2(9Z,12Z)); LysoPE(18:0/0:0); 1-heptadecanoyl-sn-glycero-3-Phosphocholine; LysoPE(0:0/20:3(5Z,8Z,11Z)); LysoPC(20:5(5Z,8Z,11Z,14Z,17Z)); Indole; Cholesterol sulfate; Acetic anhydride; 2-Hydroxyvaleric acid; Enadenine; 2-Aminoethylarsonic acid; 3-Oxotetradecanoic acid; 6-Keto-prostaglandin F1a; trans-Hexadec-2-enoyl carnitine; N-Stearoyl tyrosine; Glycerophospho-N-palmitoyl ethanolamine; LysoPE(22:6(4Z,7Z,10Z,13Z,16Z,19Z)/0:0); LysoPC(24:1(15Z)/0:0); |
