Study M592
Study name | Chen CC 2020a |
Title | Studies on the potential link between antidepressant effect of Xiaoyao San and its pharmacological activity of hepatoprotection based on multi-platform metabolomics |
Overall design | The aim of this study was to elucidate the potential link between the antidepressant and hepatoprotective effect of Xiaoyao San (XYS). A depression rat model was established by the CUMS (chronic unpredictable mild stress) procedure. The antidepressant effect of XYS was assessed by the behavioral indicators, and the hepatoprotective effect of XYS was evaluated through biochemical assays. 1H-NMR and LC/MS-based liver metabolomics were performed to discover key metabolic pathways involved in the antidepressant and hepatoprotective effects of XYS. Sprague-Dawley rats were divided into the following 5 groups (n = 10 in each group): (1) control group, (2) CUMS group, (3) CUMS + high dose of XYS group (stressor plus XYS treatment at the dose of 46 g/kg), (4) CUMS + low dose of XYS group (stressor plus XYS treatment at the dose of 23 g/kg), and (5) CUMS + venlafaxine group (stressor plus venlafaxine treatment at the dose of 35 mg/kg). The CUMS stress procedure lasted for 4 weeks, and drugs were administered via intragastric once per day during the model building period. Eight samples per group were used for LC-MS-based and NMR-based metabonomic analysis. |
Study Type | Type1; Type2; |
Data available | Unavailable |
Organism | Rat; Sprague-Dawley rat; |
Categories of depression | Animal model; Chronic mild stress model; Chronic mild stress model; |
Criteria for depression | Sucrose preference test, forced swimming test |
Sample size | 40 |
Tissue | Peripheral; Liver; Liver; |
Platform | MS-based; LC-MS: Thermo-Fisher Dionex UltiMate 3000 UHPLC-Q Exactive Orbitrap-MS (Thermo Fisher, USA); NMR; NMR: Bruker 600 MHz AVANCE III NMR spectrometer; |
PMID | |
DOI | |
Citation | Chen C, Yin Q, Tian J, et al. Studies on the potential link between antidepressant effect of Xiaoyao San and its pharmacological activity of hepatoprotection based on multi-platform metabolomics. J Ethnopharmacol. 2020;249:112432. |
Metabolite | Citric acid; L-Tryptophan; L-Valine; L-Glutamic acid; Arachidonic acid; L-Isoleucine; L-Methionine; L-Lactic acid; L-Glutamine; L-Lysine; Succinic acid; L-Tyrosine; L-Arginine; Pantothenic acid; Oxoglutaric acid; Ascorbic acid; Fumaric acid; Uracil; Creatine; Beta-D-Glucose; Niacinamide; Ornithine; L-Acetylcarnitine; Phosphorylcholine; Oxidized glutathione; Carbamoyl phosphate; Glycocholic acid; Glutathione; Glycoursodeoxycholic acid; Taurochenodesoxycholic acid; |
