Study M570
Study name | Wang LS 2019 |
Title | LC-MS/MS-based quantification of tryptophan metabolites and neurotransmitters in the serum and brain of mice |
Overall design | In this study, a simple, rapid, sensitive and specific liquid chromatography-tandem mass spectrometry method was developed for simultaneous determination of tryptophan, kynurenine, kynurenic acid, 3-hydroxykynurenine, 5-hydroxytryptamine, 5-hydroxyindoleacetic acid, norepinephrine, L-glutamic acid, GABA and acetylcholine in mice serum and the brain tissues in a single chromatographic run. In order to apply this method, the biological samples in normal control and chronic mild stress (CMS) mice were analyzed. BALB/c mice were divided into the following 2 groups: (1) control group, and (2) CMS group. The CMS stress procedure lasted for 6 weeks. Seven to nine samples per group were used for metabonomic analysis. |
Study Type | Type1; |
Data available | Unavailable |
Organism | Mouse; BALB/c mouse; |
Categories of depression | Animal model; Chronic mild stress model; Chronic mild stress model; |
Criteria for depression | Sucrose preference test, tail suspension test |
Sample size | 16 |
Tissue | Central; Brain; Hippocampus; Peripheral; Blood; Serum; Central; Brain; Cerebral cortex; |
Platform | MS-based; LC-MS: SHIMADZU Prominence LC system (Kyoto, Japan) with QTRAP 5500 mass spectrometer (AB SCIEX, Foster City, CA); |
PMID | |
DOI | |
Citation | Wang LS, Zhang MD, Tao X, et al. LC-MS/MS-based quantification of tryptophan metabolites and neurotransmitters in the serum and brain of mice. J Chromatogr B Analyt Technol Biomed Life Sci. 2019;1112:24-32. |
Metabolite | Gamma-Aminobutyric acid; L-Tryptophan; |
