Study M541
Study name | Gong WX 2019 |
Title | The anti-depression effect of Angelicae Sinensis Radix is related to the pharmacological activity of modulating the hematological anomalies |
Overall design | The aim of this study was to elucidate the potential anti-depression mechanisms of Angelicae Sinensis Radix (AS) that are related to the activity of modulating the blood system. The model of chronic unpredictable mild stress (CUMS) was performed to assess the anti-depression effect of AS. A metabolomic approach based on UPLC-MS/MS combined with 1H NMR was employed to analyze the endogenous metabolites and metabolic pathways regulated by AS in depression. Sprague-Dawley rats were divided into the following 5 groups (n = 12 in each group): (1) control group, (2) CUMS group, (3) CUMS + venlafaxine group (stressor plus venlafaxine treatment at the dose of 35 mg/kg), (4) CUMS + low dose of AS group (stressor plus AS treatment at the dose of 7.5g herb/kg), and (5) CUMS + high dose of AS group (stressor plus AS treatment at the dose of 15g herb/kg). The CUMS stress procedure lasted for 4 weeks, and drugs were administered via intragastric once per day during the model building period. |
Study Type | Type1; Type2; |
Data available | Unavailable |
Organism | Rat; Sprague-Dawley rat; |
Categories of depression | Animal model; Chronic mild stress model; Chronic mild stress model; |
Criteria for depression | Sucrose preference test, forced swimming test |
Sample size | 45 |
Tissue | Peripheral; Blood; Serum; |
Platform | MS-based; LC-MS: Dionex UltiMate 3000 UHPLC system combined with a Q Exactive Orbitrap-MS spectrometer and a Xcalibur workstation (Thermo Fisher Scientific Inc., Waltham, MA, United States); NMR; NMR: Bruker 600 MHz AVANCE III spectrometer (Bruker Biospin, Rheinstetten, Germany); |
PMID | |
DOI | |
Citation | Gong W, Zhu S, Chen C, et al. The anti-depression effect of Angelicae Sinensis Radix is related to the pharmacological activity of modulating the hematological anomalies. Front Pharmacol. 2019;10:192. |
Metabolite | Citric acid; 3-Hydroxybutyric acid; L-Tryptophan; L-Valine; L-Leucine; L-Proline; L-Glutamic acid; Glycine; L-Isoleucine; L-Methionine; L-Lactic acid; L-Alanine; L-Glutamine; Choline; Cholic acid; LysoPC(16:0); Phytosphingosine; Propionylcarnitine; Indoxyl sulfate; Taurine; Pyruvic acid; Creatine; Beta-D-Glucose; Trimethylamine N-oxide; alpha-D-Glucose; Lipid; Acetone; Indoleacrylic acid; Oleamide; Pipecolic acid; Allantoin; Octadecanamide; Deoxycholic acid; |
