Study M539
Study name | Geng CM 2019a |
Title | UPLC-Q-TOF-MS profiling of the hippocampus reveals metabolite biomarkers for the impact of Dl-3-n-butylphthalide on the lipopolysaccharide-induced rat model of depression |
Overall design | The aim of this study was to employ an ultra-high-performance LC-MS (UPLC-MS) metabolomic approach combined with principal component analysis, partial least squares-discriminate analysis, and orthogonal partial least-squares discriminant analysis to identify differentially expressed hippocampus metabolites in the lipopolysaccharide (LPS)-induced rat model of depression. Sprague-Dawley rats were divided into the following 3 groups (n = 6 in each group): (1) control group, (2) LPS group, and (3) LPS + Dl-3-n-butylphthalide group (LPS plus Dl-3-n-butylphthalide treatment). LPS was administrated every other day and was carried out 14 days, and Dl-3-n-butylphthalide (30 mg/kg/day) was administered via oral gavage during the model building period. |
Study Type | Type1; Type2; |
Data available | Unavailable |
Organism | Rat; Sprague-Dawley rat; |
Categories of depression | Animal model; Lipopolysaccharide induced depression model; Lipopolysaccharide induced depression model; |
Criteria for depression | Sucrose preference test, forced swimming test |
Sample size | 18 |
Tissue | Central; Brain; Hippocampus; |
Platform | MS-based; LC-MS: Waters Acquity UPLC system was coupled with the Waters Xevo Q-TOF mass analyzer; |
PMID | |
DOI | |
Citation | Geng C, Guo Y, Qiao Y, et al. UPLC-Q-TOF-MS profiling of the hippocampus reveals metabolite biomarkers for the impact of Dl-3-n-butylphthalide on the lipopolysaccharide-induced rat model of depression. Neuropsychiatr Dis Treat. 2019;15:1939-1950. |
Metabolite | Gamma-Aminobutyric acid; Citric acid; L-Tryptophan; L-Threonine; L-Methionine; L-Glutamine; L-Lysine; L-Arginine; Oxoglutaric acid; Xanthine; Glucose 6-phosphate; L-Histidine; Cyclic AMP; Betaine aldehyde; Glycerophosphocholine; D-Ribose 5-phosphate; CDP; Cytosine; Purine; Cinnamic acid; Quinic acid; Proline betaine; Mannitol; 2E,6E,8E-Decatrienoic acid; Octadecanedioic acid; Indole; L-Thyronine; |
