Study M486
Study name | Tian JS 2018 |
Title | Metabolomics studies on corticosterone-induced PC12 cells: a strategy for evaluating an in vitro depression model and revealing the metabolic regulation mechanism |
Overall design | In order to investigate the underlying metabolic profiles of corticosterone (CORT)-induced PC12 cells and evaluate the suitable differentiated types of PC12 cells for use in depressive studies, proton nuclear magnetic resonance (1H NMR) metabolomics coupled with network analysis approaches were employed. PC12 cells depression model was induced by CORT. Three types of differentiated (un-, poorly- and well-differentiated) PC12 cells were divided into 2 groups (6 dishes in each group): (1) control group (no drug), (2) CORT group (PC12 cells treated with corticosterone 500 umol for 36 h). |
Study Type | Type1; |
Data available | Unavailable |
Organism | Rat; Rat; |
Categories of depression | Animal model; Other animal model; Other animal model; |
Criteria for depression | Not reported |
Sample size | 12 |
Tissue | Central; Brain; PC12 cell; Central; Brain; PC12 cell medium; |
Platform | NMR; NMR: Bruker 600-MHz AVANCE III NMR spectrometer (Bruker, Germany); |
PMID | |
DOI | |
Citation | Tian JS, Liu SB, He XY, et al. Metabolomics studies on corticosterone-induced PC12 cells: a strategy for evaluating an in vitro depression model and revealing the metabolic regulation mechanism. Neurotoxicol Teratol. 2018;69:27-38. |
Metabolite | myo-Inositol; Pyroglutamic acid; L-Leucine; L-Serine; L-Isoleucine; |
