Study M207
Study name | Zheng P 2016c |
Title | Gut microbiome remodeling induces depressive-like behaviors through a pathway mediated by the host's metabolism |
Overall design | In this study, we initially assessed how gut microbiota physiologically influence the psychobehavioral characteristics of germ-free (GF) and SPF mice. To assess whether alterations of gut microbiota have a causal role in depression-like behavior, gut microbiome remodeling was accomplished through fecal microbiota transplantation (FMT) from either major depressive disorder patients or healthy individuals on GF mice followed by behavioral testing to assess depression-like behaviors. Metabolomic analyses of samples from the mice harboring 'depression microbiota' were conducted to examine how the gut microbiome influences host metabolism. GF Mice were divided into the following 2 groups (n = 10 in each group): (1) control group (fecal microbiota transplantation with fecal sample from healthy controls), (2) depressed group (fecal microbiota transplantation with fecal sample from major depression patients). On week 2 post FMT, the depressed and control mice were killed, and cecum, serum and hippocampus samples were obtained. These samples were subsequently extracted and analyzed by gas chromatography-mass spectrometry, liquid chromatography-mass spectrometry, and nuclear magnetic resonance. |
Study Type | Type1; |
Data available | Unavailable |
Organism | Mouse; Kunming mouse; |
Categories of depression | Animal model; Other animal model; Other animal model; |
Criteria for depression | Forced swimming test, tail suspension test |
Sample size | 20 |
Tissue | Peripheral; Faece; Faece; Central; Brain; Hippocampus; Peripheral; Blood; Serum; |
Platform | MS-based; GC-MS: Agilent 7890A/5975C (Agilent Technologies, Santa Clara, CA, USA); MS-based; LC-MS: Agilent 6538 UHD and Accurate-Mass Q-TOF/MS (Agilent Technologies, Santa Clara, CA, USA; NMR; NMR: Bruker AVANCE II 600 (Bruker Biospin, Rheinstetten, Germany); |
PMID | |
DOI | |
Citation | Zheng P, Zeng B, Zhou C, et al. Gut microbiome remodeling induces depressive-like behaviors through a pathway mediated by the host's metabolism. Mol Psychiatry 2016;21(6):786-96. |
Metabolite | myo-Inositol; Citric acid; Linoleic acid; Glycerol; Pyroglutamic acid; Oleic acid; Stearic acid; Myristic acid; Palmitic acid; N-Acetyl-L-aspartic acid; L-Phenylalanine; L-Leucine; L-Glutamic acid; Glycine; L-Threonine; Arachidonic acid; L-Methionine; L-Lactic acid; L-Asparagine; L-Aspartic acid; L-Lysine; Tyramine; 4-Aminohippuric acid; Succinic acid; D-Fructose; Sebacic acid; D-Arabinose; Threonic acid; Phytosphingosine; Ethanolamine; Taurine; Uracil; Pyruvic acid; Beta-D-Glucose; Trimethylamine N-oxide; Indolelactic acid; Cholesterol; alpha-D-Glucose; Acetoacetic acid; Glycolic acid; Trimethylamine; L-Malic acid; L-Carnitine; Adenine; 5-Aminopentanoic acid; Glycerol 3-phosphate; Glutathione; Allantoin; Palmitic amide; Cer(d18:1/16:0); Glycerol 1-hexadecanoate; PC(13:0); Beta-Glycerophosphoric acid; Lactose; Linolenic Acid ethyl ester; Methyl Ricinoleate; PGF2alpha Dimethyl Amide; Dihydrotachysterol; Glucoheptonic Acid; Cadaverine; 15S-Hydroxy-9-oxo-methyl ester-prosta-5Z,10,13E-trien-1-oic acid; 2-hydroxypalmitate; 7-Hexadecenoic acid, methyl ester; |
