Study M131
Study name | Liu LX 2018 |
Title | Hippocampal metabolic differences implicate distinctions between physical and psychological stress in four rat models of depression |
Overall design | The aim of the present study was to investigate the metabolic changes in the hippocampus in the four stress models of depression using a non-targeted gas chromatography-mass spectrometry (GC-MS) approach. We investigated this hypothesis by exploring hippocampal metabolic changes in four animal models of depression induced by different stress. Rats were, respectively, exposed to chronic unpredictable mild stress (CUMS), inescapable foot-shock stress (learned helplessness, LH), repeated restraint stress (chronic restraint stress, CRS) and resident-intruder stress (social defeat, SD) to mimic different types of stress in humans. In Experiment 1, the rats were divided into (1) control group (n = 8) and (2) CUMS group (n = 20). In Experiment 2, the rats were divided into (3) control group (n = 8) and (4) LH group (n = 20). In Experiment 3, the rats were divided into (5) control group (n = 10) and (6) CRS group (n = 20). In Experiment 4, the rats were divided into (7) control group (n = 8) and (8) SD group (n = 20). All stress of these models procedure lasted for 3 weeks. Only susceptible rats were used for metabolomics analysis, resulting in 68 rats (CUMS/Control = 9/8, LH/Control = 10/8, CRS/Control = 8/8, and SD/Control = 9/8). |
Study Type | Type1; |
Data available | Unavailable |
Organism | Rat; Sprague-Dawley rat; |
Categories of depression | Animal model; Chronic restraint stress model; Chronic restraint stress model; Animal model; Learned helplessness model; Learned helplessness model; Animal model; Social defeat model; Social defeat model; Animal model; Chronic mild stress model; Chronic mild stress model; |
Criteria for depression | Sucrose preference test, forced swimming test |
Sample size | 68 |
Tissue | Central; Brain; Hippocampus; |
Platform | MS-based; GC-MS: Agilent 7890A/5975C GC/MSD System (Agilent Technologies Inc., USA); |
PMID | |
DOI | |
Citation | Liu L, Zhou X, Zhang Y, et al. Hippocampal metabolic differences implicate distinctions between physical and psychological stress in four rat models of depression.Transl Psychiatry 2018;8(1):4. |
Metabolite | myo-Inositol; Gamma-Aminobutyric acid; Glycerol; Pyroglutamic acid; N-Acetyl-L-aspartic acid; L-Cysteine; L-Proline; L-Glutamic acid; Glycine; L-Serine; L-Threonine; Arachidonic acid; L-Methionine; L-Lactic acid; L-Alanine; L-Asparagine; L-Glutamine; L-Aspartic acid; L-Lysine; Succinic acid; Hypoxanthine; Ascorbic acid; Ethanolamine; Taurine; Creatine; D-Ribose; Inosine; Cholesterol; 3-Phosphoglyceric acid; Adenosine; Oxamate; 2,3-Dihydroxypyridine; Aminomalonic acid; Phosphate; O-Phosphoethanolamine; L-Allothreonine; Lipoamide; Fosfomycin; Methyl palmitoleate; N-Methylhydantoin; MG(0:0/16:0/0:0); Aminooxyacetic acid; |
