Study M1108

Study name

Zhou MF 2023b

Title

Microbiome and tryptophan metabolomics analysis in adolescent depression: roles of the gut microbiota in the regulation of tryptophan-derived neurotransmitters and behaviors in human and mice

Overall design

The aim of this study was to investigate whether Roseburia Intestinalis. colonization could ameliorate depressive behaviors in the chronic restraint stress (CRS) model. C57/6 J mice were divided into the following 4 groups: (1) control group (non-stress with PBS treatment), (2) Roseburia Intestinalis. group (non-stress with Roseburia Intestinalis. treatment), (3) CRS group (stress with PBS treatment), and (4) CRS + Roseburia Intestinalis. group (stress with Roseburia Intestinalis. treatment). Mice in the CRS groups were stressed for 14 consecutive days. Then mice in the Roseburia Intestinalis. groups were orally administered with Roseburia Intestinalis. at a dose of 200 uL/per mouse for 14 consecutive days. The kynurenine pathway metabolites in the prefrontal cortex, colon, and serum were determined using ultra-high performance liquid chromatography tandem mass spectrometry (n =4-6/group).

Study Type

Type1;

Type2;

Type3;

Data available

Unavailable

Organism

Mouse; C57/6 J mouse;

Categories of depression

Animal model; Chronic restraint stress model; Chronic restraint stress model;

Healthy individuals; Healthy individuals; Healthy individuals;

Criteria for depression

Sucrose preference test, tail suspension test, forced swimming test

Sample size

24

Tissue

Peripheral; Gut; Colon;

Peripheral; Blood; Serum;

Central; Brain; Prefrontal cortex;

Platform

MS-based; LC-MS: not reported;

PMID

37386523

DOI

10.1186/s40168-023-01589-9

Citation

Zhou M, Fan Y, Xu L, et al. Microbiome and tryptophan metabolomics analysis in adolescent depression: roles of the gut microbiota in the regulation of tryptophan-derived neurotransmitters and behaviors in human and mice. Microbiome. 2023 Jun 30;11(1):145.

Metabolite

L-Tryptophan;

5-Hydroxyindoleacetic acid;

L-Kynurenine;

Kynurenic acid;

Serotonin;

Hydroxykynurenine;

N-Acetylserotonin;

NAD;

Quinolinic acid;

3-Hydroxyanthranilic acid;