Study M1107
Study name | Zhou MF 2023a |
Title | Microbiome and tryptophan metabolomics analysis in adolescent depression: roles of the gut microbiota in the regulation of tryptophan-derived neurotransmitters and behaviors in human and mice |
Overall design | The aim of this study was to investigate whether the salutary effects of the targeted microbiota on anti-depressive behaviors in mice involving the metabolism of the tryptophan-derived neurotransmitters along the gut-brain axis. Fecal microbiota transplantation (FMT) of adolescent depression and healthy controls were performed in the chronic restraint stress (CRS) model. C57/6 J mice were divided into the following 4 groups: (1) control group, (2) CRS group, (3) CRS + FMT of adolescent depression group, and (4) CRS + FMT of healthy controls group. Mice in the CRS groups were stressed for 14 consecutive days. Mice in the FMT groups received fecal suspension from patients with adolescent depression or healthy controls once every 2 days for 14 days by oral gavage. The kynurenine pathway metabolites in the prefrontal cortex, colon, and serum were determined using ultra-high performance liquid chromatography tandem mass spectrometry (n =4-6/group). |
Study Type | Type1; Type2; |
Data available | Unavailable |
Organism | Mouse; C57/6 J mouse; |
Categories of depression | Animal model; Other animal model; Other animal model; Animal model; Chronic restraint stress model; Chronic restraint stress model; |
Criteria for depression | Sucrose preference test, tail suspension test, forced swimming test |
Sample size | 24 |
Tissue | Central; Brain; Prefrontal cortex; Peripheral; Gut; Colon; Peripheral; Blood; Serum; |
Platform | MS-based; LC-MS: not reported; |
PMID | |
DOI | |
Citation | Zhou M, Fan Y, Xu L, et al. Microbiome and tryptophan metabolomics analysis in adolescent depression: roles of the gut microbiota in the regulation of tryptophan-derived neurotransmitters and behaviors in human and mice. Microbiome. 2023 Jun 30;11(1):145. |
Metabolite | L-Tryptophan; 5-Hydroxyindoleacetic acid; L-Kynurenine; Kynurenic acid; |
