Study M1087

Study name

Xie J 2023b

Title

Tryptophan metabolism as bridge between gut microbiota and brain in chronic social defeat stress-induced depression mice

Overall design

The aim of this study was to explore the relationships between gut microbiota and host’s metabolism in the chronic social defeat stress (CSDS) model of depression. C57BL/6 mice were divided into the following 2 groups: (1) control group, and (2) CSDS group. Fecal samples were collected from CSDS group and control group to measure gut microbiota and microbial metabolites. Eight samples per group were used for metabonomic analysis. Tryptophan metabolism-related metabolites in hippocampus were also analyzed.

Study Type

Type1;

Data available

Unavailable

Organism

Mouse; C57BL/6 mouse;

Categories of depression

Animal model; Social defeat model; Social defeat model;

Criteria for depression

Sucrose preference test, forced swimming test, social interaction test

Sample size

16

Tissue

Peripheral; Faece; Faece;

Central; Brain; Hippocampus;

Platform

MS-based; LC-MS: Agilent 1290 infinity LC ultra high performance liquid chromatography system with AB 6500 qtrap mass spectrometer (AB SCIEX);

PMID

36909723

DOI

10.3389/fcimb.2023.1121445

Citation

Xie J, Wu WT, Chen JJ, et al. Tryptophan metabolism as bridge between gut microbiota and brain in chronic social defeat stress-induced depression mice. Front Cell Infect Microbiol. 2023 Feb 24;13:1121445.

Metabolite

L-Tryptophan;

Arachidonic acid;

Uridine;

Serotonin;

Glucose 6-phosphate;

Acetic acid;

N-Acetyl-L-glutamic acid;

Docosahexaenoic acid;

Stearoylcarnitine;

Glycerol 3-phosphate;

LysoPC(P-16:0);

Valeric acid;

Cortisol;

Propionic acid;

5-Hydroxy-L-tryptophan;

20-Hydroxyeicosatetraenoic acid;

LysoPC(14:0/0:0);

Ceanothenic acid;

13-HDoHE;

Pyrroline hydroxycarboxylic acid;

Glycerophosphoinositol;

LysoPE(20:1(11Z)/0:0);

Phenobarbital;

4-Hydroxy-3-methoxy-cinnamoylglycine;

D-myo-Inositol 4-phosphate;

LysoPE(18:1(11Z)/0:0);

2-Pyrrolidineacetic acid;

Valylproline;

Prostaglandin A1;

Cysteine-S-sulfate;

Homocitrulline;

5b-Cholestane-3a,7a,12a,23S,25-pentol;

PC(P-18:0/0:0);

PC(16:0/0:0);

Ganoderic acid L;

Neuromedin N (1-4);

Neuraminic acid;

p-Mentha-1,3,5,8-tetraene;

cis-3-Hexenyl trans-2-hexenoate;

PA(O-16:0/0:0);

PE(P-16:0/0:0);

Hydroxybuprenorphine;

Orotidine;

Verbenalin;

Epicatechin 7-sulfate;

Aldosterone 18-glucuronide;

1-Deoxy-D-xylulose;

PE(P-20:0/0:0);

2',2'-Difluorodeoxyuridine;

Ramipril Diketopiperazine;

5-Deoxykievitone hydrate;

xi-2,3-Dihydro-3,5-dihydroxy-6-methyl-4H-pyran-4-one;

Gibberellin A95;

Ethyl (S)-3-hydroxybutyric acid glucoside;

12-Hydroxynevirapine glucuronide;

N-Acetylglucosamine 6-sulfate;

4a-Methylzymosterol-4-carboxylic acid;

Vanillin acetate;

PE(P-18:0/0:0);

11-Hydroxyeicosatetraenoate glyceryl ester;

Epoxydocosapentaenoic acid;

Blumealactone C;

dCMP;

(1E,4Z,6a,8b,10a)-8-Angeloyloxy-10,15-dihydroxy-3-oxo-1,4,11(13)-germacratrien-12,6-olide;

N-Lactoylphenylalanine;

Arginylproline;

Homoveratric acid;

(E)-2-Penten-1-ol;

Succinyldisalicylic acid;

Austalide L;

Isorhamnetin 3-beta-D-glucoside;

Kainic Acid;

Apigenin 7-arabinoside;

LysoPA(20:3n6/0:0);

Coumestan;

Isovalerylalanine;

Cinnamyl alcohol;

2,3-Dimethyl-2-cyclohexen-1-one;

2,6-Di-tert-butyl-4-methylene-2,5-cyclohexadienone;

(3b,6b,8a,12a)-8,12-Epoxy-7(11)-eremophilene-6,8,12-trimethoxy-3-ol;

5-Hexyltetrahydro-2-oxo-3-furancarboxylic acid;

4,6-Decadiyn-1-ol;

6-Phenyl-3-hexen-2-one;

2-Hydrazinopyridine;

24-Hydroxycalcitriol;

cis-3-Hexenyl tiglate;

hydroxyoctadecadienoic acid;

2,2,6,7-Tetramethylbicyclo[4.3.0]nona-1(9),4-dien-8-one;

9-oxo-11-(3-Pentyloxiran-2-yl)undec-10-enoate;

Dihydroxyeicosatrienoic acid;

DiMe(3,5);

16-B1-phytoprostane;

Abscisic alcohol;

Cepagenin;

Leukotriene B4 ethanolamide;

Cinncassiol E;

Ethyl (2E,4E,7Z)-decatrienoic acid;

L-Citronellol glucoside;

3-Hydroxy-C4-HSL;

2-(3-Hydroxy-4-methylphenyl)-5-methyl-4-hexen-3-one;

Apo-10'-violaxanthal;

H-D-Asp(OtBu)-OH;

PE(17:0/0:0);

10-Hydroxy-2,8-decadiene-4,6-diynoic acid;

beta-Costic acid;

3-(2-Methylpropanoyloxy)-8-(2-methylbutanoyloxy)-9,10-epoxy-p-mentha-1,3,5-triene;

2,3-Dimethyl-3-hydroxyglutaric acid;

Octadeca-2,4-dienoic acid;

3,4-DHPEA-EA;

Gibberellin A37;

Iodotyrosine;

5-Methyldeoxycytidine;

(1R*,3R*,3'S*)-1,2,3,4-Tetrahydro-1-(2-thio-3-pyrrolidinyl)-beta-carboline-3-carboxylic acid;

(Z)-Narceine imide;

15-Octadecene-9,11,13-triynoic acid;

Isomucronulatol;

2-Methoxyestrone;

L-Threoneopterin;

PE(24:1(15Z)/24:1(15Z));

Bilobalide A;

3b-Allotetrahydrocortisol;