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Study M107

Study name

He XY 2017

Title

[Comparative study of the corticosterone and glutamate induced PC12 cells depression model by 1H NMR metabolomics]

Overall design

This study was designed to analyze the change of metabolites in the PC12 cells and its medium induced by corticosterone (CORT) and glutamate (Glu) by proton nuclear magnetic resonance (1H NMR) metabolomics. PC12 cells depression model was induced by CORT and Glu. PC12 cells were divided into 3 groups (6 dishes in each group): (1) control group (no drug), (2) CORT group (PC12 cells treated with corticosterone 500 umol/L for 36 h), and (3) Glu group (PC12 cells treated with glutamate 20 mmol/L for 36 h).

Study Type

Type1;

Data available

Unavailable

Organism

Rat; Rat;

Categories of depression

Animal model; Other animal model; Other animal model;

Criteria for depression

Not reported

Sample size

18

Tissue

Central; Brain; PC12 cell;

Central; Brain; PC12 cell medium;

Platform

NMR; NMR: Bruker AVANCE III 600 spectrometer (Bruker Biospin, Rheinstetten, Germany);

PMID

N/A

DOI

10.16438/j.0513-4870.2016-0543

Citation

He X, Chen J, Xiang H, et al. [Comparative study of the corticosterone and glutamate induced PC12 cells depression model by 1H NMR metabolomics]. Yao Xue Xue Bao 2017;52(2):245-52. (Article in Chinese)

Metabolite

myo-Inositol;

Glycerol;

Pyroglutamic acid;

3-Hydroxybutyric acid;

N-Acetyl-L-aspartic acid;

L-Phenylalanine;

L-Valine;

L-Leucine;

L-Glutamic acid;

Glycine;

L-Serine;

L-Isoleucine;

L-Lactic acid;

L-Alanine;

L-Asparagine;

L-Aspartic acid;

L-Lysine;

Succinic acid;

L-Tyrosine;

Choline;

L-Cystine;

Taurine;

Pyruvic acid;

Creatine;

Beta-D-Glucose;

Acetic acid;

L-Histidine;

alpha-D-Glucose;

Ornithine;

Dimethylamine;

Phosphorylcholine;

Glycerophosphocholine;

Glutathione;

Methylamine;

2-Oxovaline;

Methylguanidine;

3-Methyl-2-oxovaleric acid;