Study M1019
Study name | Bian XQ 2022a |
Title | Identification of proline, 1-pyrroline-5-carboxylate and glutamic acid as biomarkers of depression reflecting brain metabolism using carboxylomics, a new metabolomics method |
Overall design | In this study, a sensitive 5-(diisopropylamino) amylamine derivatization coupled with UHPLC-Q-TOF/MS was used to profile carboxylic acids in serum samples of patients with depression. A total of 130 serum samples, including 65 patients with depression (depression group) and 65 healthy controls (control group) were recruited. The training cohort included 19 patients with depression and 15 healthy controls, and the validation cohort included 46 patients with depression and 50 healthy controls. Metabolic profiling was performed based on the samples from the training cohort, then potential biomarkers were validated in the validation cohort. The difference with P < 0.05 was considered as statistical significance. |
Study Type | Type1; |
Data available | Unavailable |
Organism | Human; |
Categories of depression | Depressive symptom; Depressive symptom; Depressive symptom; |
Criteria for depression | HAMD-17 |
Sample size | 130 |
Tissue | Peripheral; Blood; Serum; |
Platform | MS-based; LC-MS: Agilent 1290 Infinity LC system (UHPLC, Santa Clara) with Agilent 6545 Q-TOF/MS system; |
PMID | |
DOI | |
Citation | Bian X, Zhou N, Zhao Y, et al. Identification of proline, 1-pyrroline-5-carboxylate and glutamic acid as biomarkers of depression reflecting brain metabolism using carboxylomics, a new metabolomics method. Psychiatry Clin Neurosci. 2023 Apr;77(4):196-204. |
Metabolite | Linoleic acid; Pyroglutamic acid; L-Phenylalanine; L-Valine; L-Proline; L-Glutamic acid; Glycine; L-Serine; L-Threonine; Arachidonic acid; L-Lactic acid; L-Aspartic acid; Caproic acid; Suberic acid; 5-Hydroxyindoleacetic acid; L-Arginine; Sebacic acid; Indoleacetic acid; Fumaric acid; L-Histidine; Docosahexaenoic acid; Isobutyrylglycine; Ornithine; L-Malic acid; Isobutyric acid; Erythronic acid; 3-Hydroxyanthranilic acid; Glutathione; Lithocholic acid; Valeric acid; Propionic acid; Alpha-Linolenic acid; N-Butyrylglycine; Picolinic acid; Nervonic acid; Docosapentaenoic acid (22n-3); Dihomo-gamma-linolenic acid; Hydroxyisovaleric acid; N-Acetylserine; Isovaleric acid; Docosatrienoic acid; 1-Pyrroline-5-carboxylic acid; But-2-enoic acid; Hydroxyoctanoic acid; Aminoisobutyric acid; Tetrahydropyridine-carboxylic acid; Aminodecanoic acid; 2-Amino-4-ethoxy-3-hydroxybutanoic acid; 2-Aminoacrylic acid; 3b-Hydroxy-5-cholenoic acid; Heptanoic acid; Hexadecadienoic acid; Docosatetraenoic acid; |
