Study M082

Study name

Liang ZH 2016

Title

GC-MS-based metabolomic study on the antidepressant-like effects of diterpene ginkgolides in mouse hippocampus

Overall design

Here, we investigated the antidepressant-like effects of diterpene ginkgolides (DG), an important class of constituents in Ginkgo biloba extract (GBE), and studied their effects in the mouse hippocampus using a GC-MS-based metabolomics approach. Mice were randomly divided into the following 5 groups: (1) control group (n = 9), (2) low dose of DG group (DGMI diluted in 0.9% NaCl solution to a dose of 4.06 mg/kg, n = 10), (3) medium dose of DG group (DGMI diluted in 0.9% NaCl solution to a dose of 12.18 mg/kg, n = 11), (4) high dose of DG group (DGMI diluted in 0.9% NaCl solution to a dose of 36.54 mg/kg, n = 11), and (5) venlafaxine group (venlafaxine diluted in 0.9% NaCl solution to a dose of 16 mg/kg, n = 11). Mice were injected intraperitoneally daily between 8:00 AM and 10:00 AM with the same amount of solution for 14 days. Behavioral testing (sucrose preference and tail suspension tests) showed the medium-dose group showed a better antidepressant-like effect, suggesting that the DG antidepressant-like effect was dose related. Samples from control group, medium dose of DG group, and venlafaxine group were used for metabolomics analysis.

Study Type

Type3;

Data available

Unavailable

Organism

Mouse; C57BL/6J mouse;

Categories of depression

Healthy individuals; Healthy individuals; Healthy individuals;

Criteria for depression

Sucrose preference test, tail suspension test

Sample size

52

Tissue

Central; Brain; Hippocampus;

Platform

MS-based; GC-MS: Agilent GC system 7890A equipped with 5975C mass selective detector system;

PMID

27498146

DOI

10.1016/j.bbr.2016.08.001

Citation

Liang Z, Bai S, Shen P, et al. GC-MS-based metabolomic study on the antidepressant-like effects of diterpene ginkgolides in mouse hippocampus. Behav Brain Res 2016;314:116-24.

Metabolite

Gamma-Aminobutyric acid;

Pyroglutamic acid;

N-Acetyl-L-aspartic acid;

L-Cysteine;

Glycine;

L-Lactic acid;

L-Alanine;

L-Asparagine;

L-Glutamine;

Kynurenic acid;

Ethanolamine;

Inosine;

Pyrophosphate;

Glycerol 3-phosphate;

Phosphate;

N-Oleoyldopamine;

O-Phosphoethanolamine;

N-Acetyl-beta-alanine;