Study M076
Study name | Du HL 2016 |
Title | Metabonomic identification of the effects of the Zhimu-Baihe saponins on a chronic unpredictable mild stress-induced rat model of depression |
Overall design | This study investigated the anti-depressant activity of the total saponins from Zhimu and Baihe and the mechanisms underlying using a chronic unpredictable mild stress (CUMS)-induced rat model of depression. Optimized pretreatment of samples and mass spectrometry conditions were used to analyze the metabonomic profile of the hippocampus. Rats were randomly divided into 6 groups: (1) control group (n = 9), (2) CUMS group (n = 12), (3) CUMS + fluoxetine group (stress plus fluoxetine treatment at the dose of 10 mg/kg, n = 12), (4) CUMS + Anemarrhena saponins group (stress plus Anemarrhena saponins treatment at the dose of 48 mg/kg, n = 12), (5) CUMS + Lilium saponins group (stress plus Lilium saponins treatment at the dose of 48 mg/kg, n = 12), and (6) CUMS + Anemarrhena saponins and Lilium saponins group (stress plus Anemarrhena saponins and Lilium saponins treatment 48 mg/kg, n = 12). 9, 11, 12, 12, 11, and 12 sample were used for metabonomic analysis in these groups. The CUMS stress procedure lasted for 6 weeks, and drugs were administered by gavage daily during the model building period. |
Study Type | Type1; Type2; |
Data available | Unavailable |
Organism | Rat; Sprague-Dawley rat; |
Categories of depression | Animal model; Chronic mild stress model; Chronic mild stress model; |
Criteria for depression | Sucrose preference test, forced swimming test |
Sample size | 67 |
Tissue | Central; Brain; Hippocampus; |
Platform | MS-based; LC-MS: Agilent 1290 Infinity liquid chromatography system which combined with Agilent 6538 Accurate Mass Quadrupole Time-of-Flight mass spectrometer (Agilent, USA); |
PMID | |
DOI | |
Citation | Du H, Wang K, Su L, et al. Metabonomic identification of the effects of the Zhimu-Baihe saponins on a chronic unpredictable mild stress-induced rat model of depression. J Pharm Biomed Anal 2016;128:469-79. |
Metabolite | Oleic acid; N-Acetyl-L-aspartic acid; L-Phenylalanine; L-Tryptophan; L-Valine; L-Leucine; L-Threonine; Arachidonic acid; L-Methionine; L-Glutamine; L-Aspartic acid; L-Tyrosine; Phenylpyruvic acid; L-Arginine; LysoPC(16:0); Hypoxanthine; Ascorbic acid; Taurine; Docosahexaenoic acid; Inosine; Phosphorylcholine; Glycerophosphocholine; D-Ribose 5-phosphate; N-Acetyl-L-alanine; Indoleacrylic acid; Phosphoenolpyruvic acid; Glutathione; LysoPE(0:0/22:6(4Z,7Z,10Z,13Z,16Z,19Z)); |
