Study M024
Study name | Gao XX 2013 |
Title | An investigation of the antidepressant action of xiaoyaosan in rats using ultra performance liquid chromatography-mass spectrometry combined with metabonomics |
Overall design | In this study, a rapid, highly sensitive, and selective method was applied in a non-invasive way to investigate the antidepressant action of Xiaoyaosan (XYS) using ultra performance liquid chromatography-mass spectrometry (UPLC-MS) and chemometrics. Urine obtained from rats subjected to chronic unpredictable mild stress (CUMS) was analyzed by UPLC-MS. Rats were divided into 6 groups (n = 8 in each group): (1) control group, (2) CUMS group, (3) CUMS + venlafaxine group (stress plus venlafaxine treatment at a dose of 25 mg/kg), (4) CUMS + fluoxetine group (stress plus fluoxetine treatment at a dose of 6 mg/kg), (5) CUMS + middle dose of XYS group (stress plus XYS treatment at a dose of 46.2 g/kg), and (6) CUMS + high dose of XYS group (stress plus XYS treatment at a dose of 92.4 g/kg). Seven samples per group were used for metabonomic analysis. The CUMS stress procedure lasted for 3 weeks, and drugs were administered via the intragastric route once a day during the model building period. |
Study Type | Type1; Type2; |
Data available | Unavailable |
Organism | Rat; Sprague-Dawley rat; |
Categories of depression | Animal model; Chronic mild stress model; Chronic mild stress model; |
Criteria for depression | Sucrose preference test |
Sample size | 42 |
Tissue | Peripheral; Urine; Urine; |
Platform | MS-based; LC-MS: Waters ACQUITY UPLC system coupled to a Waters ACQUITY TQD mass spectrometer (Waters Corporation, Milford, MA, USA); |
PMID | |
DOI | |
Citation | Gao XX, Cui J, Zheng XY, et al. An investigation of the antidepressant action of xiaoyaosan in rats using ultra performance liquid chromatography-mass spectrometry combined with metabonomics. Phytother Res 2013;27(7):1074-85. |
Metabolite | Citric acid; L-Phenylalanine; L-Tryptophan; Hippuric acid; Succinic acid; L-Tyrosine; Kynurenic acid; N2-Succinyl-L-ornithine; Phenylacetylglycine; Xanthurenic acid; Creatinine; Indoleacetic acid; Oxoglutaric acid; |
