Study M018
Study name | Wang XY 2012 |
Title | Metabonomics approach to assessing the modulatory effects of St John's wort, ginsenosides, and clomipramine in experimental depression |
Overall design | The aim of this study was to evaluate therapeutic effects of antidepressants comprehensively in the chronic unpredictable mild stress (CUMS)-induced depression model by means of metabolite profiling along with classic pharmacological and biochemical indices. The protective effects of St John's Wort extract (SJ), ginsenosides (GS), and clomipramine (CPM) on chronic unpredictable mild stress (CUMS)-induced depression in rats were investigated by using a combination of behavioral assessments and metabonomics. Metabonomic analyses of plasma, serum, and brains were performed using gas chromatography/mass spectrometry. Rats were randomly divided into the following five groups (n = 6 in each group): (1) control group, (2) CUMS group, (3) CUMS + St John's Wort extract group (stress plus St John's Wort extract treatment at the dose of 1 g/kg), (4) CUMS + ginsenosides group (stress plus ginsenosides treatment at the dose of 100 mg/kg), and (5) CUMS + clomipramine group (stress plus clomipramine treatment at the dose of 50 mg/kg). The CUMS stress procedure lasted for 4 weeks, and drugs were administered once a day during the first 25 days of model building period. |
Study Type | Type1; Type2; |
Data available | Unavailable |
Organism | Rat; Sprague-Dawley rat; |
Categories of depression | Animal model; Chronic mild stress model; Chronic mild stress model; |
Criteria for depression | Sucrose preference test |
Sample size | 30 |
Tissue | Peripheral; Blood; Serum; Central; Brain; Brain; Peripheral; Urine; Urine; |
Platform | MS-based; GC-MS: Agilent 6890N g chromatograph coupled with a Pegasus HT time-of-flight mass spectrometer (GC/TOF, Leco Corp., St. Joseph, MI); MS-based; GC-MS: hyphenated Perkin-Elmer gas chromatograph and TurboMass coupled to an Autosystem XL mass spectrometer (PerkinElmer, USA); |
PMID | |
DOI | |
Citation | Wang X, Zeng C, Lin J, et al. Metabonomics approach to assessing the modulatory effects of St John's wort, ginsenosides, and clomipramine in experimental depression. J Proteome Res 2012;11(12):6223-30. |
Metabolite | Citric acid; Pyroglutamic acid; Palmitic acid; N-Acetyl-L-aspartic acid; L-Phenylalanine; L-Cysteine; L-Tryptophan; L-Valine; L-Leucine; L-Proline; L-Glutamic acid; Glycine; L-Serine; Arachidonic acid; L-Methionine; L-Lactic acid; L-Alanine; L-Glutamine; L-Aspartic acid; L-Lysine; Homovanillic acid; Hippuric acid; Tyramine; 4-Aminohippuric acid; Pimelic acid; Succinic acid; Caproic acid; Suberic acid; L-Tyrosine; 5-Hydroxyindoleacetic acid; D-Arabinose; Spermine; Creatinine; Gamma-Tocopherol; Fumaric acid; Ethanolamine; Taurine; Pyruvic acid; N-Acetyl-L-glutamic acid; N-Acetylglutamine; Indolelactic acid; Hypotaurine; 4-Hydroxybutyric acid; L-Histidine; Niacinamide; 4-Hydroxyproline; |
