Study M975
Study name | Liang XQ 2022 |
Title | Integrated 16S rRNA sequencing and metabolomics analysis to investigate the antidepressant role of Yang-Xin-Jie-Yu decoction on microbe-gut-metabolite in chronic unpredictable mild stress-induced depression rat model |
Overall design | The aim of this study was to investigate the underlying antidepressant mechanisms of Yang-Xin-Jie-Yu Decoction in chronic unpredictable mild stress (CUMS)-induced depression rat model. Sprague-Dawley rats were divided into the following 6 groups (n = 15 in each group): (1) control group, (2) CUMS group, (3) CUMS + fluoxetine group (stressor plus fluoxetine treatment at the dose of 2.1 mg/kg), (4) CUMS + low dose of Yang-Xin-Jie-Yu Decoction group (stressor plus Yang-Xin-Jie-Yu Decoction treatment at the dose of 0.525 g/kg), (5) CUMS + middle dose of Yang-Xin-Jie-Yu Decoction group (stressor plus Yang-Xin-Jie-Yu Decoction treatment at the dose of 1.05 g/kg), and (6) CUMS + high dose of Yang-Xin-Jie-Yu Decoction group (stressor plus Yang-Xin-Jie-Yu Decoction treatment at the dose of 2.1 g/kg). The CUMS stress procedure lasted for 5 weeks, and drugs were administered via intragastric during the model building period. Urine, serum, and cecum content samples were used for NMR-based metabonomic analysis. |
Study Type | Type1; Type2; |
Data available | Unavailable |
Organism | Rat; Sprague-Dawley rat; |
Categories of depression | Animal model; Chronic mild stress model; Chronic mild stress model; |
Criteria for depression | Sucrose preference test, forced swimming test |
Sample size | 36 |
Tissue | Peripheral; Urine; Urine; Peripheral; Blood; Serum; Peripheral; Faece; Faece; |
Platform | NMR; NMR: Bruker 600 MHz AVANCE III NMR instrument (Karlsruhe, Germany); |
PMID | |
DOI | |
Citation | Liang XQ, Mai PY, Qin H, et al. Integrated 16S rRNA sequencing and metabolomics analysis to investigate the antidepressant role of Yang-Xin-Jie-Yu decoction on microbe-gut-metabolite in chronic unpredictable mild stress-induced depression rat model. Front Pharmacol. 2022 Sep 30;13:972351. |
Metabolite | Pyroglutamic acid; L-Isoleucine; Hippuric acid; Succinic acid; Dimethylglycine; Taurine; 1-Methylhistidine; L-Malic acid; |
