Study M942

Study name

Ge PY 2022

Title

Berberine ameliorates depression-like behavior in CUMS mice by activating TPH1 and inhibiting IDO1-associated with tryptophan metabolism

Overall design

The aim of this study was to investigate the potential role of berberine in regulating tryptophan metabolism and subsequently ameliorating depressive behavior in the chronic unpredictable mild stress (CUMS) model of depression. C57BL/6 mice were divided into the following 3 groups (n = 10 in each group): (1) control group, (2) CUMS group, and (3) CUMS + berberine group (stressor plus berberine treatment at the dose of 10 mg/kg). The CUMS stress procedure lasted for 4 weeks, and berberine was administered via intragastric once per day for 1 week after stress. The levels of neurotransmitters in the hippocampus, prefrontal cortex, striatum, and amygdala tissues were evaluated by LC-MS/MS. Then hippocampal, serum, and fecal samples were collected for metabolomics analysis. Ten samples per group were used for metabonomic analysis.

Study Type

Type1;

Type2;

Data available

Unavailable

Organism

Mouse; C57BL/6 mouse;

Categories of depression

Animal model; Chronic mild stress model; Chronic mild stress model;

Criteria for depression

Forced swimming test

Sample size

30

Tissue

Central; Brain; Hippocampus;

Central; Brain; Prefrontal cortex;

Central; Brain; Striatum;

Peripheral; Blood; Serum;

Peripheral; Faece; Faece;

Central; Brain; Amygdala;

Platform

MS-based; LC-MS: Agilent 5,500 Q-trap LC/MS (Agilent Technologies, Palo Alto, USA) system;

MS-based; LC-MS: Agilent 5,600 Q-TOF LC/MS (Agilent Technologies, Palo Alto, USA) system;

PMID

36089660

DOI

10.1002/ptr.7616

Citation

Ge PY, Qu SY, Ni SJ, et al. Berberine ameliorates depression-like behavior in CUMS mice by activating TPH1 and inhibiting IDO1-associated with tryptophan metabolism. Phytother Res. 2022 Sep 11.

Metabolite

Gamma-Aminobutyric acid;

L-Tryptophan;

L-Glutamic acid;

5-Hydroxyindoleacetic acid;

L-Kynurenine;

Serotonin;

Hydroxykynurenine;

Dopamine;

Melatonin;

LysoPC(15:0/0:0);

Acetylcholine;

LysoPC(18:0/0:0);

PC(14:0/20:1(11Z));

Indole-3-carboxaldehyde;

LysoPC(18:1(11Z)/0:0);

PC(18:1(11Z)/16:1(9Z));