Study M911

Study name

Ling XW 2022

Title

Effects of Chang-Kang-Fang formula on the microbiota-gut-brain axis in rats with irritable bowel syndrome

Overall design

The aim of this study was to investigate the in vivo effects of Chang-Kang-Fang formula in alleviating irritable bowel syndrome and depression-like behavior caused due to chronic unpredictable stress (CUS). Sprague-Dawley rats were divided into the following 3 groups (n = 8 in each group): (1) control group, (2) CUS group, and (3) CUS + Chang-Kang-Fang formula group (stressor plus Chang-Kang-Fang formula treatment at the dose of 5.0 g/kg). The CUS stress procedure lasted for 3 weeks, and drugs were administered via intragastric once per day during the model building period. Six serum samples per group were used for metabolic profiling.

Study Type

Type1;

Data available

Unavailable

Organism

Rat; Sprague-Dawley rat;

Categories of depression

Animal model; Chronic mild stress model; Chronic mild stress model;

Criteria for depression

Sucrose preference test, forced swimming test

Sample size

18

Tissue

Peripheral; Blood; Serum;

Platform

MS-based; LC-MS: not reported;

PMID

35614949

DOI

10.3389/fphar.2022.778032

Citation

Ling X, Peng S, Zhong J, et al. Effects of Chang-Kang-Fang formula on the microbiota-gut-brain axis in rats with irritable bowel syndrome. Front Pharmacol. 2022 May 9;13:778032.

Metabolite

Gamma-Aminobutyric acid;

Pyroglutamic acid;

Palmitic acid;

N-Acetyl-L-aspartic acid;

L-Glutamic acid;

L-Serine;

Homovanillic acid;

L-Tyrosine;

Azelaic acid;

Uridine;

L-Cystine;

Pseudouridine;

Taurocholic acid;

Serotonin;

Ascorbic acid;

Taurine;

2-Hydroxybutyric acid;

Gluconic acid;

Cyclic AMP;

1-Methylhistidine;

Acetoacetic acid;

Indoleacetaldehyde;

Glyceraldehyde 3-phosphate;

Adenosine;

Allantoic acid;

Adenosine monophosphate;

Maleic acid;

Carbamoyl phosphate;

Adenine;

Sorbitol;

Isobutyric acid;

D-Mannose;

5-Aminopentanoic acid;

Lithocholic acid;

Benzoic acid;

Guanosine;

Cortisol;

Methionine sulfoxide;

D-Arabitol;

p-Aminobenzoic acid;

Carnosine;

4-Hydroxycinnamic acid;

Ortho-Hydroxyphenylacetic acid;

Acetylcysteine;

3-Methyl-2-oxovaleric acid;

Mannitol;

4-Hydroxyphenylpyruvic acid;

Acetylcholine;

13-HODE;

Dodecanoic acid;

Phosphoglycolic acid;

Anserine;

4-Hydroxybenzoic acid;

D-Ornithine;

Undecanoic acid;

3-Methylthiopropionic acid;

N-Formyl-L-methionine;

Mevalonic acid-5P;

D-Glucuronic acid;

m-Cresol;

Formylanthranilic acid;

Erucic acid;

Caffeine;

Itaconic acid;

Indole-3-carboxaldehyde;

Chavicol;

4-Guanidinobutanoic acid;

1-Pyrroline-2-carboxylic acid;

Beta-Leucine;

2(3H)-Benzothiazolethione;

1-Hexadecanol;

Se-Methylselenocysteine;

N-Carbamoylputrescine;

Ethirimol;

Maleamic acid;

2-Amino-2-deoxy-D-gluconic acid;

Kojic acid;

3-Dehydroecdysone;

Methyl sulfate;

Nicotine;

Alanyltryptophan;

Acetylcholine chloride;

Styrene Oxide;

L-Arogenate;

(3S,5S)-Carbapenam-3-carboxylic acid;

epsilon-Caprolactone;

Methyloxaloacetate;

Nalidixic Acid;

L-Prolinamide;

3-Methyladenine;

Carvedilol;

2-Hydroxylaminobenzoic acid;

D-threo-3-hydroxyaspartate;

(1R,6S)-6-Amino-5-oxocyclohex-2-ene-1-carboxylate;

3,4-dihydroxyphenylpyruvate;

L-Olivosyl-oleandolide;

cis,cis-Muconic acid;

Scopoletin;

L-5-Carboxymethylhydantoin;

N-Methyltyramine;

D-beta-Phenylalanine;

4-Hydroxyphenylacetaldehyde;

Cinnamaldehyde;

6-Methylmercaptopurine;

Perillyl aldehyde;

2,4'-Dihydroxyacetophenone;

3-Amino-4-hydroxybenzoic acid;

gamma-Glutamyl-beta-aminopropiononitrile;

Eugenol;

(4S)-4-hydroxy-2,3,4,5-tetrahydro-(2S)-dipicolinate;

Aminosalicylic Acid;

Isoxaben;

Porphobilinogen;

Pseudouridine 5'-phosphate;

Hydroquinone;

1-Rhamnono-1,4-lactone;

Timolol;

2,6-Dimethoxyphenol;