Study M870
Study name | Ji S 2022 |
Title | Jia Wei Xiao Yao San ameliorates chronic stress-induced depression-like behaviors in mice by regulating the gut microbiome and brain metabolome in relation to purine metabolism |
Overall design | The aim of this study was to explore the effects of Jia Wei Xiao Yao San on chronic stress-induced depression-like behaviors in mice. A chronic restraint stress (CRS) mouse model of depression was established. Jia Wei Xiao Yao San was administered, and the responses of these mice to treatment were evaluated through several behavioral tests. C57BL/6 mice were divided into the following 4 groups: (1) control group, (2) CRS group, (3) CRS + sertraline group (stressor plus sertraline treatment at the dose of 10 mg/kg), and (4) CRS + middle dose of Jia Wei Xiao Yao San group (stressor plus Jia Wei Xiao Yao San treatment at the dose of 2 g/kg). During 8 consecutive weeks of experiments, stressed mice placed in a well-ventilated transparent restrainer for 3 h once daily. Drugs were administered via intragastric once per day during the model building period. Liquid chromatography-mass spectrometry and gas chromatography-mass spectrometry-based untargeted metabolomics was performed to investigate metabolic brain functions (n = 9 /group). |
Study Type | Type1; Type2; |
Data available | Unavailable |
Organism | Mouse; C57BL/6 mouse; |
Categories of depression | Animal model; Chronic restraint stress model; Chronic restraint stress model; |
Criteria for depression | Sucrose preference test, forced swimming test |
Sample size | 36 |
Tissue | Central; Brain; Brain; |
Platform | MS-based; GC-MS: GC instrument 7890A (Agilent Technologies) coupled with a single Q mass spectrometer 5975C (Agilent Technologies); MS-based; LC-MS: Aquity UPLC (Waters, Milford, MA, USA) coupled to a triple TOF5600 plus mass spectrometer detector (MS, Applied Biosystems, Foster City, CA); |
PMID | |
DOI | |
Citation | Ji S, Han S, Yu L, et al. Jia Wei Xiao Yao San ameliorates chronic stress-induced depression-like behaviors in mice by regulating the gut microbiome and brain metabolome in relation to purine metabolism. Phytomedicine. 2022 Jan 15;98:153940. |
Metabolite | Palmitic acid; N-Acetyl-L-aspartic acid; L-Phenylalanine; L-Glutamic acid; L-Threonine; L-Isoleucine; L-Aspartic acid; Oxalic acid; D-Glucose; Taurocholic acid; Threonic acid; Urea; Phytosphingosine; Hydroxylamine; Glucose 6-phosphate; Creatine; Niacinamide; Gluconic acid; Inosine; Xanthosine; Behenic acid; Glucose 1-phosphate; Adenosine; Maleic acid; Adenine; 3-Aminoisobutanoic acid; Oleoylcarnitine; Stearoylcarnitine; Heptadecanoic acid; Aminomalonic acid; Putrescine; Dodecanoylcarnitine; Sedoheptulose; Tetradecanoylcarnitine; Guanidoacetic acid; PG(34:1); Phosphate; 11Z-Eicosenoic acid; L-Palmitoylcarnitine; D-Arabitol; Sphingosine; L-Norleucine; Cer(d18:1/18:0); PI(38:6); PI(40:6); PS(36:1); PS(38:4); PS(40:7); PS(40:6); PS(40:4); PI(36:4); PI(38:5); PI(38:4); Glucoheptonic Acid; 3-Hydroxymethylglutaric acid; Sphinganine; Phosphoglycolic acid; Glutaral; Oxamic acid; Cortexolone; d-Glucoheptose; PG(36:2); Androsterone; PG(44:12); PG(38:5); PS(44:12); Isopropyl-beta-D-thiogalactopyranoside; 4-Hydroxylphenyllactic acid; 19-Hydroxyandrost-4-ene-3,17-dione; N-carbobenzyloxyl-leucine; Canrenone; 3-Hydroxynorvaline; Nebularine; |
