Study M820
Study name | Kim SS 2021 |
Title | UPLC-MS/MS-based profiling of 31 neurochemicals in the mouse brain after treatment with the antidepressant nefazodone |
Overall design | In this study, the authors developed a method for simultaneous analysis of 31 neurochemicals in mouse whole brain tissue using liquid chromatography tandem mass spectrometry (LC-MS/MS). ICR mice were divided into the following 4 groups: (1) single-dose control group (single-dose vehicle treatment), (2) single-dose nefazodone group (single-dose of nefazodone), (3) multiple-dose control group (vehicle treatment for three doses), and (4) multiple-dose nefazodone group (nefazodone treatment for three doses, every 2 h one dose). Nefazodone was administered intraperitoneally injected into mouse at a dose of 100 mg/kg. Brain tissues were collected 1 hour after injection. Five samples per group were used for targeted metabonomic analysis. |
Study Type | Type3; |
Data available | Unavailable |
Organism | Mouse; ICR mouse; |
Categories of depression | Healthy individuals; Healthy individuals; Healthy individuals; |
Criteria for depression | Not reported |
Sample size | 20 |
Tissue | Central; Brain; Brain; |
Platform | MS-based; LC-MS: ultra-performance liquid chromatograph (ACQUITY UPLCTM system, Waters Corporation, Milford, MA, USA) with Xevo TQ-S triple quadrupole mass spectrometer (Waters Corporation); |
PMID | |
DOI | |
Citation | Kim SS, Lee H, Song JS, et al. UPLC-MS/MS-based profiling of 31 neurochemicals in the mouse brain after treatment with the antidepressant nefazodone. Microchemical Journal. 2021, 169, 106580. |
Metabolite | Gamma-Aminobutyric acid; L-Phenylalanine; L-Leucine; L-Glutamic acid; L-Tyrosine; 5-Hydroxyindoleacetic acid; Choline; L-Kynurenine; |
