Study M815
Study name | Zhou YZ 2021 |
Title | Study on antidepressant mechanism of Radix Bupleuri-Radix Paeoniae Alba herb pair by metabonomics combined with 1H nuclear magnetic resonance and ultra-high-performance liquid chromatography-tandem mass spectrometry detection technology |
Overall design | The aim of this study was to verify the antidepressant effect of Radix Bupleuri-Radix Paeoniae Alba by behavioural examination, and reveal the underlying antidepressant mechanisms of Radix Bupleuri-Radix Paeoniae Alba. The antidepressant effects in chronic unpredictable mild stress (CUMS) of Radix Bupleuri-Radix Paeoniae Alba were observed by behavioural indicators and 1H nuclear magnetic resonance (1H-NMR) and ultra-high-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) metabonomics techniques combined with the related analysis platforms. Sprague-Dawley rats were divided into the following 5 groups: (1) control group, (2) CUMS group, (3) CUMS + venlafaxine group (stressor plus venlafaxine treatment at the dose of 35 mg/kg), (4) CUMS + low dose of Radix Bupleuri-Radix Paeoniae Alba group (stressor plus Radix Bupleuri-Radix Paeoniae Alba treatment at the dose of 7.5 g/kg), and (5) CUMS + high dose of Radix Bupleuri-Radix Paeoniae Alba group (stressor plus Radix Bupleuri-Radix Paeoniae Alba treatment at the dose of 15 g/kg). The CUMS stress procedure lasted for 4 weeks, and drugs were administered via intragastric once per day during the model building period. Seven serum samples per group were used for metabonomic analysis. |
Study Type | Type1; Type2; |
Data available | Unavailable |
Organism | Rat; Sprague-Dawley rat; |
Categories of depression | Animal model; Chronic mild stress model; Chronic mild stress model; |
Criteria for depression | Sucrose preference test, forced swimming test |
Sample size | 35 |
Tissue | Peripheral; Blood; Serum; |
Platform | NMR; NMR: BRUKER 600 MHz AVANCE III spectrometer; MS-based; LC-MS: Dionex UltiMate 3000 UHPLC system with the Q Exactive Orbitrap-MS spectrometer; |
PMID | |
DOI | |
Citation | Zhou Y, Li T, Zhu S, et al. Study on antidepressant mechanism of Radix Bupleuri-Radix Paeoniae Alba herb pair by metabonomics combined with 1H nuclear magnetic resonance and ultra-high-performance liquid chromatography-tandem mass spectrometry detection technology. J Pharm Pharmacol. 2021 Apr 22:rgab061. |
Metabolite | Citric acid; L-Leucine; L-Glutamic acid; L-Isoleucine; L-Alanine; L-Glutamine; LysoPC(16:0); Serotonin; Pyruvic acid; Betaine; |
