Study M809
Study name | Su ZH 2021 |
Title | Combining 1H-NMR-based metabonomics and network pharmacology to dissect the mechanism of antidepression effect of Milletia speciosa Champ on mouse with chronic unpredictable mild stress-induced depression |
Overall design | In this study, a proton nuclear magnetic resonance (1H-NMR)-based metabonomics combined network pharmacology research approach was performed to investigate the antidepressive mechanism of Milletia speciosa Champ act on mouse with chronic unpredictable mild stress (CUMS)-induced depression. Kunming mice were divided into the following 4 groups (n = 10 in each group): (1) control group, (2) CUMS group, (3) CUMS + Milletia speciosa Champ group (stressor plus Milletia speciosa Champ treatment at the dose of 20 g/kg), and (4) CUMS + fluoxetine group (stressor plus fluoxetine treatment at the dose of 20 mg/kg). The CUMS stress procedure lasted for 5 weeks, and drugs were administered via intragastric once per day during the model building period. 1H-NMR-based metabonomics and network pharmacology were used to screen serum biomarkers of CUMS-induced depression and investigate the therapeutic mechanism of Milletia speciosa Champ. |
Study Type | Type1; Type2; |
Data available | Unavailable |
Organism | Mouse; Kunming mouse; |
Categories of depression | Animal model; Chronic mild stress model; Chronic mild stress model; |
Criteria for depression | Sucrose preference test |
Sample size | 40 |
Tissue | Peripheral; Blood; Serum; |
Platform | NMR; NMR: 600 MHz NMR spectrometer (Varian INOVA, Varian, Inc., USA); |
PMID | |
DOI | |
Citation | Su Z, Ruan J, Liu X, et al. Combining 1H-NMR-based metabonomics and network pharmacology to dissect the mechanism of antidepression effect of Milletia speciosa Champ on mouse with chronic unpredictable mild stress-induced depression. J Pharm Pharmacol. 2021 Jun 8;73(7):881-892. |
Metabolite | L-Phenylalanine; L-Tryptophan; L-Isoleucine; |
