Study M787
Study name | Li CC 2021 |
Title | Chronic restraint stress induced changes in colonic homeostasis-related indexes and tryptophan-kynurenine metabolism in rats |
Overall design | The aim of this study was to examine whether chronic restraint stress (CRS) could initiate and aggravate colonic inflammation, integrity damage and metabolic disturbance of rats. Wistar rats were divided into the following 2 groups: (1) control group, and (2) CRS group. The CRS stress procedure lasted for 4 weeks, and rats were placed in well-ventilated Plexiglas tubes from 9 am to 15 pm every day. For the colon samples, untargeted metabolomics analysis based on ultra-performance liquid chromatography coupled with quadrupole time-of-flight mass was performed, and tryptophan metabolites were quantified via prominence ultrafast liquid chromatography coupled with a QTRAP 5500 mass. Eight samples per group were used for metabonomic analysis. |
Study Type | Type1; |
Data available | Unavailable |
Organism | Rat; Wistar rat; |
Categories of depression | Animal model; Chronic restraint stress model; Chronic restraint stress model; |
Criteria for depression | Sucrose preference test, forced swimming test |
Sample size | 16 |
Tissue | Peripheral; Gut; Colon; |
Platform | MS-based; LC-MS: UPLC-Q-TOF/MS; MS-based; LC-MS: UFLC-Q-TRAP 5500 (AB SCIEX, Framingham, MA, USA); |
PMID | |
DOI | |
Citation | Li CC, Gan L, Tan Y, et al. Chronic restraint stress induced changes in colonic homeostasis-related indexes and tryptophan-kynurenine metabolism in rats. J Proteomics. 2021 May 30;240:104190. |
Metabolite | Glutaric acid; L-Tryptophan; Succinic acid; 5-Hydroxyindoleacetic acid; L-Kynurenine; Chenodeoxycholic acid; Kynurenic acid; Hypoxanthine; Hydroxykynurenine; Indole-3-carboxylic acid; Adenine; 3-Hydroxyanthranilic acid; Muricholic acid; Kynurenic acid/3-Hydroxykynurenine ratio; Indole; LysoPE(0:0/16:1(9Z)); |
