Study M771
Study name | Gong X 2021 |
Title | Altered fecal metabolites and colonic glycerophospholipids were associated with abnormal composition of gut microbiota in a depression model of mice |
Overall design | In this study, we established a chronic social defeated stress (CSDS) mice model of depression and applied multi-omics techniques (16S rRNA gene sequencing, non-target metabolomics, and lipidomics) to investigate the associations between the gut microbiota and gastrointestinal tract. C57BL/6J mice were divided into the following 2 groups: (1) control group, and (2) CSDS group (social defeated stress for 10 days). We applied 16S rRNA gene sequencing to assess the gut microbial composition and gas and liquid chromatography-mass spectroscopy to identify fecal metabolites and colonic lipids, respectively (n = 10/8, control/CSDS for metabolomic and lipid profiling). |
Study Type | Type1; |
Data available | Unavailable |
Organism | Mouse; C57BL/6J mouse; |
Categories of depression | Animal model; Social defeat model; Social defeat model; |
Criteria for depression | Sucrose preference test, forced swimming test, social interaction test, tail suspended test |
Sample size | 18 |
Tissue | Peripheral; Faece; Faece; Peripheral; Gut; Colon; |
Platform | MS-based; LC-MS: UPLC system (Nexera LC-30A, SHIMADZU) equipped with Q-Exactive Plus (Thermo Scientific); MS-based; GC-MS: GC7890 system coupled to MSD5975 mass selective detector (Agilent, California, USA); |
PMID | |
DOI | |
Citation | Gong X, Huang C, Yang X, et al. Altered fecal metabolites and colonic glycerophospholipids were associated with abnormal composition of gut microbiota in a depression model of mice. Front Neurosci. 2021 Jul 19;15:701355. |
Metabolite | Gamma-Aminobutyric acid; Stearic acid; Myristic acid; L-Methionine; Behenic acid; Heptadecanoic acid; 11Z-Eicosenoic acid; Arachidic acid; 3-Methyl-2-oxovaleric acid; 4-Hydroxybenzoic acid; PE(18:0p/18:1)+H; PE(18:0/18:2)-H; SM(d40:1)+H; PC(36:2e)+H; PE(16:0p/22:5)-H; PC(16:0/16:0)+HCOO; PE(18:0p/20:3)+H; PE(36:3p)-H; N-Dodecane; Heptadecane; Tricosane; 3,5-Diiodo-L-tyrosine; 2-Hydroxyglutaramic acid; Nonadecanoic acid; Tricosanoic acid; 10-Heptadecenoic acid; 10-Pentadecenoic acid; (9E,11E)-Octadecadienoic acid; PE(20:0p/18:2)+H; PE(18:0/18:1)+Na; LysoPC(18:0)+H; PC(37:2)+H; PE(38:2p)-H; PE(18:0p/18:2)+H; PC(16:0p/18:2)+HCOO; PC(34:2p)+H; CerG1(d20:0+pO/24:0+O)+H; PC(33:2)+H; PE(16:0p/18:2)+H; PC(18:0p/18:1)+H; PC(18:1/18:1)+HCOO; PC(16:0e/18:2)+HCOO; PE(20:0p/18:1)-H; PG(22:6/22:6)-H; PE(16:0p/20:5)+H; PC(16:0/20:4)+HCOO; PE(20:0p/20:5)-H; PE(18:0p/22:6)-H; SM(d42:3)+H; |
