Study M722
Study name | Siopi E 2020b |
Title | Changes in gut microbiota by chronic stress impair the efficacy of fluoxetine |
Overall design | In this study, the authors used the chronic unpredictable mild stress (CUMS) model of depression to study the effect of gut microbiota on the antidepressant and neurogenic effects of fluoxetine. C57BL/6J mice were divided into the following 4 groups: (1) control-transferred group (microbiota recipient mice, transfer from control mice), (2) CUMS-transferred group (microbiota recipient mice, transfer from CUMS mice), (3) CUMS-transferred + 5-hydroxytryptophan group (microbiota recipient mice, transfer from CUMS mice, with 5-hydroxytryptophan treatment at the dose of 100 mg/kg), and (4) CUMS-transferred + fluoxetine + 5-hydroxytryptophan group (microbiota recipient mice, transfer from CUMS mice, with both fluoxetine and 5-hydroxytryptophan treatment, at the dose of 18 and 100 mg/kg, respectively). The CUMS stress procedure lasted for 8 weeks, and drugs were administered via water during the last 4 weeks of the model building period. Five serum, hippocampus, and gut samples per group were used for metabonomic analysis. |
Study Type | Type1; Type2; |
Data available | Unavailable |
Organism | Mouse; C57BL/6J mouse; |
Categories of depression | Animal model; Other animal model; Other animal model; |
Criteria for depression | Forced swimming test, tail suspension test |
Sample size | 20 |
Tissue | Peripheral; Blood; Serum; Central; Brain; Hippocampus; Peripheral; Gut; Gut; |
Platform | MS-based; GC-MS: Thermo-Finnigan Trace DSQ fast-scanning single-quadrupole mass spectrometer; MS-based; LC-MS: Waters ACQUITY UPLC and Thermo-Finnigan LTQ mass spectrometer; |
PMID | |
DOI | |
Citation | Siopi E, Chevalier G, Katsimpardi L, et al. Changes in gut microbiota by chronic stress impair the efficacy of fluoxetine. Cell Rep. 2020;30(11):3682-3690.e6. |
Metabolite | L-Phenylalanine; L-Tryptophan; L-Valine; L-Proline; L-Isoleucine; |
